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dc.contributor.authorLo, Chih Hungen_US
dc.contributor.authorZeng, Jialiuen_US
dc.identifier.citationLo, C. H. & Zeng, J. (2022). Application of polymersomes in membrane protein study and drug discovery: progress, strategies, and perspectives. Bioengineering and Translational Medicine, 8(1), 1-33.
dc.description.abstractMembrane proteins (MPs) play key roles in cellular signaling pathways and are responsible for intercellular and intracellular interactions. Dysfunctional MPs are directly related to the pathogenesis of various diseases, and they have been exploited as one of the most sought-after targets in the pharmaceutical industry. However, working with MPs is difficult given that their amphiphilic nature requires protection from biological membrane or membrane mimetics. Polymersomes are bilayered nano-vesicles made of self-assembled block copolymers that have been widely used as cell membrane mimetics for MP reconstitution and in engineering of artificial cells. This review highlights the prevailing trend in the application of polymersomes in MP study and drug discovery. We begin with a review on the techniques for synthesis and characterization of polymersomes as well as methods of MP insertion to form proteopolymersomes. Next, we review the structural and functional analysis of the different types of MPs reconstituted in polymersomes, including membrane transport proteins, MP complexes, and membrane receptors. We then summarize the factors affecting reconstitution efficiency and the quality of reconstituted MPs for structural and functional studies. Additionally, we discuss the potential in using proteopolymersomes as platforms for high-throughput screening (HTS) in drug discovery to identify modulators of MPs. We conclude by providing future perspectives and recommendations on advancing the study of MPs and drug development using proteopolymersomes.en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.relation.ispartofBioengineering and Translational Medicineen_US
dc.rights© 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_US
dc.titleApplication of polymersomes in membrane protein study and drug discovery: progress, strategies, and perspectivesen_US
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.description.versionPublished versionen_US
dc.subject.keywordsBiophysical Characterizationen_US
dc.subject.keywordsDrug Discoveryen_US
dc.description.acknowledgementThis study is supported by a Lee Kong Chian School of Medicine Dean's Postdoctoral Fellowship to Chih Hung Lo (Grant Award Number 021207-00001) from Nanyang Technological University (NTU) Singapore. This study is also supported by a Presidential Postdoctoral Fellowship to Jialiu Zeng (Grant Award Number 021229-00001) from NTU Singapore.en_US
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