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Title: Shotgun metagenomic sequencing analysis of ocular surface microbiome in Singapore residents with mild dry eye
Authors: Tong, Louis
Constancias, Florentin
Hou, Aihua
Chua, Song Lin
Drautz-Moses, Daniela Isabel
Schuster, Stephan Christoph
Yang, Liang
Williams, Rohan B. H.
Kjelleberg, Staffan
Keywords: Science::Medicine
Issue Date: 2022
Source: Tong, L., Constancias, F., Hou, A., Chua, S. L., Drautz-Moses, D. I., Schuster, S. C., Yang, L., Williams, R. B. H. & Kjelleberg, S. (2022). Shotgun metagenomic sequencing analysis of ocular surface microbiome in Singapore residents with mild dry eye. Frontiers in Medicine, 9, 1034131-.
Project: NMRC/CSA/045/2012 
Journal: Frontiers in Medicine 
Abstract: The ocular surface microbiome has implications for ocular surface inflammation and immunology. Previous shotgun metagenomics analyses were performed in China, showing results that differed according to environment and age. Patients with Sjogren's syndrome were reported to have altered conjunctival microbiome, but such studies have not been done in milder dry eye. The aim of this study is to describe the conjunctival microbiome in people with mild dry eye in Singapore. Samples were collected from 14 participants with mild dry eye and 10 age-matched comparison participants recruited from Singapore National Eye Centre (SNEC) clinics. Shotgun metagenomic sequencing analysis was employed to evaluate the conjunctival microbiome composition. Proteobacteria formed the predominant phylum in the conjunctiva. As in a study from a coastal city in China, Achromobacter spp. was numerically most abundant. Compared to age-matched controls, the conjunctival microbial composition in mild dry eye was similar. Several microorganisms, including Streptococcus spp. increased in representation with age, and the abundance of Staphylococcus correlated with Schirmer readings. In addition, when cultured corneal epithelial cells were exposed to three strains of Achromobacter xylosoxidans, cytokines such as TNF-α and IL-6 were upregulated in the cell lysates and supernatants. Ourresults suggest that age is an important factor that affects composition of the conjunctival microbiome, and relative abundance of specific microorganism may vary according to the environment of the human host.
ISSN: 2296-858X
DOI: 10.3389/fmed.2022.1034131
Schools: Lee Kong Chian School of Medicine (LKCMedicine) 
School of Biological Sciences 
Organisations: Life Sciences Institute, NUS
Research Centres: Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) 
Rights: © 2022 Tong, Constancias, Hou, Chua, Drautz-Moses, Schuster, Yang, Williams and Kjelleberg. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
SBS Journal Articles
SCELSE Journal Articles

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