Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/164515
Title: Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers
Authors: Li, Di
Xiong, Qirong
Liu, Wylie
Liang, Li
Duan, Hongwei
Keywords: Science::Chemistry
Issue Date: 2023
Source: Li, D., Xiong, Q., Liu, W., Liang, L. & Duan, H. (2023). Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers. Biosensors and Bioelectronics, 219, 114795-. https://dx.doi.org/10.1016/j.bios.2022.114795
Project: MOE2018-T2-2-128 
Journal: Biosensors and Bioelectronics 
Abstract: Nanozymes with enzyme-mimicking catalytic activity and unique functions have stimulated increasing interest in the biosensing field. Herein, we report a magnetic nanozyme (MNE) with integrated superior peroxidase-like activity and efficient mixing ability. This nanozymatic magnetic nanomixer is synthesized by depositing a Fe2+-doped polydopamine coating on the surface of well-aligned magnetic nanoparticles to form a rigid chain-like nanostructure. Polydopamine coating of the nanozymatic MNE allows for efficient immobilization of natural enzymes such as glucose oxidase, cholesterol oxidase or urate oxidase to produce a series of enzymes-immobilized MNE (MNE@enzymes) with intrinsic multienzyme cascade properties. These MNE@enzymes show synchronously rotating capability in spinning magnetic fields, which leads to an 80∼100% improvement in their overall catalytic efficiencies. In the on-chip detection of small molecular metabolites (i.e., glucose, cholesterol, and uric acid), the rotating MNE@enzymes lead to detection sensitivities 2.1∼4.3 times higher than those of the static ones. Importantly, the consistent performance of the rotating MNE@enzymes offers the possibility of integrating the detection of glucose, free cholesterol and uric acid into a single multiplexing microchip assay with smartphone readout, affording an improved sensitivity, good selectivity and reliability. The designed enzymes-loaded MNEs holds great promise in developing rapid and ultrasensitive measurements of diverse targets of healthcare concerns using portable devices.
URI: https://hdl.handle.net/10356/164515
ISSN: 0956-5663
DOI: 10.1016/j.bios.2022.114795
Schools: School of Chemistry, Chemical Engineering and Biotechnology 
Rights: © 2022 Elsevier B.V. A. All rights reserved. This paper was published in Biosensors and Bioelectronics and is made available with permission of Elsevier B.V.
Fulltext Permission: embargo_20250108
Fulltext Availability: With Fulltext
Appears in Collections:CCEB Journal Articles

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