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Title: Monochloramine induces release of DNA and RNA from bacterial cells: quantification, sequencing analyses, and implications
Authors: Bairoliya, Sakcham
Goel, Apoorva
Mukherjee, Manisha
Koh, Jonas Zhi Xiang
Cao, Bin
Keywords: Engineering::Civil engineering
Issue Date: 2022
Source: Bairoliya, S., Goel, A., Mukherjee, M., Koh, J. Z. X. & Cao, B. (2022). Monochloramine induces release of DNA and RNA from bacterial cells: quantification, sequencing analyses, and implications. Environmental Science & Technology, 56(22), 15791-15804.
Journal: Environmental Science & Technology
Abstract: Monochloramine (MCA) is a widely used secondary disinfectant to suppress microbial growth in drinking water distribution systems. In monochloraminated drinking water, a significant amount of extracellular DNA (eDNA) has been reported, which has many implications ranging from obscuring DNA-based drinking water microbiome analyses to posing potential health concerns. To address this, it is imperative for us to know the origin of the eDNA in drinking water. Using Pseudomonas aeruginosa as a model organism, we report for the first time that MCA induces the release of nucleic acids from both biofilms and planktonic cells. Upon exposure to 2 mg/L MCA, massive release of DNA from suspended cells in both MilliQ water and 0.9% NaCl was directly visualized using live cell imaging in a CellASIC ONIX2 microfluidic system. Exposing established biofilms to MCA also resulted in DNA release from the biofilms, which was confirmed by increased detection of eDNA in the effluent. Intriguingly, massive release of RNA was also observed, and the extracellular RNA (eRNA) was also found to persist in water for days. Sequencing analyses of the eDNA revealed that it could be used to assemble the whole genome of the model organism, while in the water, certain fragments of the genome were more persistent than others. RNA sequencing showed that the eRNA contains non-coding RNA and mRNA, implying its role as a possible signaling molecule in environmental systems and a snapshot of the past metabolic state of the bacterial cells.
ISSN: 0013-936X
DOI: 10.1021/acs.est.2c06632
Schools: School of Civil and Environmental Engineering 
Interdisciplinary Graduate School (IGS) 
Research Centres: Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) 
Rights: © 2022 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:CEE Journal Articles
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