Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/164632
Title: Lipid-coated hybrid nanoparticles for enhanced bacterial biofilm penetration and antibiofilm efficacy
Authors: Lee, Hiang Wee
Kharel, Sharad
Loo, Joachim Say Chye
Keywords: Engineering::Materials
Engineering::Bioengineering
Issue Date: 2022
Source: Lee, H. W., Kharel, S. & Loo, J. S. C. (2022). Lipid-coated hybrid nanoparticles for enhanced bacterial biofilm penetration and antibiofilm efficacy. ACS Omega, 7(40), 35814-35824. https://dx.doi.org/10.1021/acsomega.2c04008
Project: MOE/RCE: M4330019.C70 
RG19/18 
RT08/19 
SNBC/2021/SF2/P04 
SFS_RND_SUFP_001_06 
Journal: ACS Omega 
Abstract: Up to 80% of all infections are biofilm-mediated and they are often challenging to treat as the underlying bacterial cells can become 100- to 1000-fold more tolerant toward antibiotics. Antibiotic-loaded nanoparticles have gained traction as a potential drug delivery system to treat biofilm infections. In particular, lipid-coated hybrid nanoparticles (LCHNPs) were investigated on their capability to deliver antibiotics into biofilms. In this study, LCHNPs composed of a poly(lactic-co-glycolic acid) (PLGA) core and dioleoyl-3-trimethylammonium propane (DOTAP) lipid shell were developed and loaded with vancomycin (Van). In vitro antibacterial and antibiofilm tests were performed to evaluate the antimicrobial efficacy of the LCHNPs. LCHNPs were successfully fabricated with high vancomycin encapsulation and loading efficiencies, and exhibited enhanced antibacterial effects against planktonic Staphylococcus aureus USA300 when compared against Free-Van and Van-PLGANPs. When used to treat USA300 biofilms, Van-LCHNPs eradicated up to 99.99% of the underlying biofilm cells, an effect which was not observed for Free-Van and Van-PLGANPs. Finally, we showed that by possessing a robust DOTAP shell, LCHNPs were able to penetrate deeply into the biofilms.
URI: https://hdl.handle.net/10356/164632
ISSN: 2470-1343
DOI: 10.1021/acsomega.2c04008
Schools: School of Materials Science and Engineering 
Lee Kong Chian School of Medicine (LKCMedicine) 
Research Centres: Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) 
Rights: © 2022 The Authors. Published by American Chemical Society. This is an open-access article distributed under the terms of the Creative Commons Attribution License.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
MSE Journal Articles
SCELSE Journal Articles

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