Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/164641
Title: 3D printing biocompatible materials with multi jet fusion for bioreactor applications
Authors: Priyadarshini, Balasankar Meera
Kok, Wai Kay
Dikshit, Vishwesh
Feng, Shilun
Li, Holden King Ho
Zhang, Yi
Keywords: Engineering::Mechanical engineering
Issue Date: 2023
Source: Priyadarshini, B. M., Kok, W. K., Dikshit, V., Feng, S., Li, H. K. H. & Zhang, Y. (2023). 3D printing biocompatible materials with multi jet fusion for bioreactor applications. International Journal of Bioprinting, 9(1), 14-35. https://dx.doi.org/10.18063/ijb.v9i1.623
Journal: International Journal of Bioprinting 
Abstract: In the evolving three-dimensional (3D) printing technology, the involvement of different materials in any new 3D printing process necessitates a thorough evaluation of the product's biocompatibility for biomedical application. Here, we examined the ability of Multi Jet Fusion (MJF)-printed PA-12 to support cell proliferation and osteogenesis. Our results show that leachate from MJF-printed PA-12 does not inhibit the growth of L929 fibroblast and MC3T3e1 osteoblast. The substrate supports the attachment and proliferation of both cell types, though not at a level comparable to conventional polystyrene culture plate. Neither plasma treatment, poly-D-lysine, nor collagen coatings narrowed the gap substantially, suggesting the possible influence of other limiting factors. The substrate can also support MC3T3e1 osteogenesis. However, MJF-printed PA-12 exhibits varying ability in supporting the proliferation of different cell types, especially in subsequent passages. While L929's proliferation is comparable from passage-to-passage, MC3T3e1's growth ability is noticeably compromised. Interestingly, our results show that L929 subcultured back to polystyrene plate retains the ability to grow as robustly as those on the conventional plate, suggesting that MJF-printed PA-12 does not permanently impair cell proliferation. In addition, we have shown the successful culture of bacterial Escherichia coli on MJF-printed PA-12. Together, our study demonstrated the potential of MJF-printed PA-12 for biological applications.
URI: https://hdl.handle.net/10356/164641
ISSN: 2424-7723
DOI: 10.18063/ijb.v9i1.623
Schools: School of Mechanical and Aerospace Engineering 
School of Electrical and Electronic Engineering 
Research Centres: HP-NTU Digital Manufacturing Corporate Lab
Rights: © 2022 Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License, permitting distribution, and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:EEE Journal Articles
MAE Journal Articles

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