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Title: Squid suckerin-spider silk fusion protein hydrogel for delivery of mesenchymal stem cell secretome to chronic wounds
Authors: Koh, Kenrick
Wang, Jun Kit
Chen, James Xiao Yuan
Hiew, Shu Hui
Cheng, Hong Sheng
Gabryelczyk, Bartosz
Vos, Marcus Ivan Gerard
Yip, Yun Sheng
Chen, Liyan
Sobota, Radoslaw M.
Chua, Damian Kang Keat
Tan, Nguan Soon
Tay, Chor Yong
Miserez, Ali
Keywords: Engineering::Materials
Issue Date: 2023
Source: Koh, K., Wang, J. K., Chen, J. X. Y., Hiew, S. H., Cheng, H. S., Gabryelczyk, B., Vos, M. I. G., Yip, Y. S., Chen, L., Sobota, R. M., Chua, D. K. K., Tan, N. S., Tay, C. Y. & Miserez, A. (2023). Squid suckerin-spider silk fusion protein hydrogel for delivery of mesenchymal stem cell secretome to chronic wounds. Advanced Healthcare Materials, 12(1), 2201900-.
Project: MOE 2018-T2-1-043
NRF-SIS “SingMass"
Journal: Advanced Healthcare Materials
Abstract: Chronic wounds are non-healing wounds characterized by a prolonged inflammation phase. Excessive inflammation leads to elevated protease levels and consequently to a decrease in growth factors at wound sites. Stem cell secretome therapy has been identified as a treatment strategy to modulate the microenvironment of chronic wounds via supplementation with anti-inflammatory/growth factors. However, there is a need to develop better secretome delivery systems that are able to encapsulate the secretome without denaturation, in a sustained manner, and that are fully biocompatible. To address this gap, a recombinant squid suckerin-spider silk fusion protein is developed with cell-adhesion motifs capable of thermal gelation at physiological temperatures to form hydrogels for encapsulation and subsequent release of the stem cell secretome. Freeze-thaw treatment of the protein hydrogel results in a modified porous cryogel that maintains slow degradation and sustained secretome release. Chronic wounds of diabetic mice treated with the secretome-laden cryogel display increased wound closure, presence of endothelial cells, granulation wound tissue thickness, and reduced inflammation with no fibrotic scar formation. Overall, these in vivo indicators of wound healing demonstrate that the fusion protein hydrogel displays remarkable potential as a delivery system for secretome-assisted chronic wound healing.
ISSN: 2192-2640
DOI: 10.1002/adhm.202201900
Schools: School of Materials Science and Engineering 
Lee Kong Chian School of Medicine (LKCMedicine) 
Interdisciplinary Graduate School (IGS) 
Research Centres: NTU Institute for Health Technologies 
Center for Sustainable Materials (SusMat)
Rights: © 2022 Wiley-VCH GmbH. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:IGS Journal Articles
LKCMedicine Journal Articles
MSE Journal Articles

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