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Title: Bio-mimicking acellular wet electrospun scaffolds promote accelerated integration and re-epithelialization of full-thickness dermal wounds
Authors: Chin, Jiah Shin
Madden, Leigh
Phillips, Anthony R. J.
Chew, Sing Yian
Becker, David Lawrence
Keywords: Science::Medicine
Issue Date: 2022
Source: Chin, J. S., Madden, L., Phillips, A. R. J., Chew, S. Y. & Becker, D. L. (2022). Bio-mimicking acellular wet electrospun scaffolds promote accelerated integration and re-epithelialization of full-thickness dermal wounds. Bioengineering, 9(7), 9070324-.
Project: H17/01/a0/0C9 
SRG 15005 
Journal: Bioengineering 
Abstract: Scaffolds can promote the healing of burns and chronic skin wounds but to date have suffered from issues with achieving full skin integration. Here, we characterise the wound response by both tissue integration and re-epithelialization to a scaffold using wet electrospinning to fabricate 3D fibrous structures. Two scaffold materials were investigated: poly(ε-caprolactone) (PCL) and PCL + 20% rat tail type 1 collagen (PCL/Coll). We assessed re-epithelisation, inflammatory responses, angiogenesis and the formation of new extracellular matrix (ECM) within the scaffolds in rat acute wounds. The 3D PCL/Coll scaffolds impeded wound re-epithelisation, inducing a thickening of wound-edge epidermis as opposed to a thin tongue of migratory keratinocytes as seen when 3D PCL scaffolds were implanted in the wounds. A significant inflammatory response was observed with 3D PCL/Coll scaffolds but not with 3D PCL scaffolds. Enhanced fibroblast migration and angiogenesis into 3D PCL scaffolds was observed with a significant deposition of new ECM. We observed that this deposition of new ECM within the scaffold was key to enabling re-epithelialization over the scaffold. Such scaffolds provide a biocompatible environment for cell integration to lay down new ECM and encourage re-epithelisation over the implanted scaffold.
ISSN: 2306-5354
DOI: 10.3390/bioengineering9070324
Schools: Interdisciplinary Graduate School (IGS) 
School of Chemical and Biomedical Engineering 
Lee Kong Chian School of Medicine (LKCMedicine) 
School of Materials Science and Engineering 
Organisations: Skin Research Institute, Singapore
Research Centres: Nanyang Institute of Health Technologies
Rights: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:IGS Journal Articles
LKCMedicine Journal Articles
MSE Journal Articles
SCBE Journal Articles

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