Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/164773
Title: Oleaginous microalga coccomyxa subellipsoidea as a highly effective cell factory for CO₂ fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen
Authors: Liu, Yu 
Wei, Dong
Chen, Weining
Keywords: Engineering::Bioengineering
Issue Date: 2022
Source: Liu, Y., Wei, D. & Chen, W. (2022). Oleaginous microalga coccomyxa subellipsoidea as a highly effective cell factory for CO₂ fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen. Frontiers in Bioengineering and Biotechnology, 10, 921024-. https://dx.doi.org/10.3389/fbioe.2022.921024
Journal: Frontiers in Bioengineering and Biotechnology 
Abstract: Microalgae used for CO2 biofixation can effectively relieve CO2 emissions and produce high-value biomass to achieve "waste-to-treasure" bioconversion. However, the low CO2 fixation efficiency and the restricted application of biomass are currently bottlenecks, limiting the economic viability of CO2 biofixation by microalgae. To achieve high-efficient CO2 fixation and high-protein biomass production, the oleaginous microalga Coccomyxa subellipsoidea (C. subellipsoidea) was cultivated autotrophically through optimizing inorganic carbon and nitrogen supply. 0.42 g L-1 NaHCO3 supplemented with 2% CO2 as a hybrid carbon source resulted in high biomass concentration (3.89 g L-1) and productivity (318.33) with CO2 fixation rate 544.21 mg L-1 d-1 in shake flasks. Then, used in a 5-L photo-fermenter, the maximal protein content (60.93% DW) in batch 1, and the highest CO2 fixation rate (1043.95 mg L-1 d-1) with protein content (58.48% DW) in batch 2 of repeated fed-batch cultures were achieved under 2.5 g L-1 nitrate. The relative expression of key genes involved in photosynthesis, glycolysis, and protein synthesis showed significant upregulation. This study developed a promising approach for enhancing carbon allocation to protein synthesis in oleaginous microalga, facilitating the bioconversion of the fixed carbon into algal protein instead of oil in green manufacturing.
URI: https://hdl.handle.net/10356/164773
ISSN: 2296-4185
DOI: 10.3389/fbioe.2022.921024
Schools: School of Chemical and Biomedical Engineering 
Rights: © 2022 Liu, Wei and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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