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Title: Chemical elicitation as an avenue for discovery of bioactive compounds from fungal endophytes
Authors: Munusamy, Madhaiyan
Ching, Kuan Chieh
Yang, Lay Kien
Crasta, Sharon
Gakuubi, Martin Muthee
Chee, Zhao Yan
Wibowo, Mario
Leong, Chung Yan
Kanagasundaram, Yoganathan
Ng, Siew Bee
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Munusamy, M., Ching, K. C., Yang, L. K., Crasta, S., Gakuubi, M. M., Chee, Z. Y., Wibowo, M., Leong, C. Y., Kanagasundaram, Y. & Ng, S. B. (2022). Chemical elicitation as an avenue for discovery of bioactive compounds from fungal endophytes. Frontiers in Chemistry, 10, 1024854-.
Project: #21719 
Journal: Frontiers in Chemistry 
Abstract: The present study investigated the molecular phylogeny, antimicrobial and cytotoxic activities of fungal endophytes obtained from the A*STAR Natural Organism Library (NOL) and previously isolated from Sungei Buloh Wetland Reserve, Singapore. Phylogenetic analysis based on ITS2 gene suggests that these isolates belong to 46 morphotypes and are affiliated to 23 different taxa in 17 genera of the Ascomycota phylum. Colletotrichum was the most dominant fungal genus accounting for 37% of all the isolates, followed by Diaporthe (13%), Phyllosticta (10.9%) and Diplodia (8.7%). Chemical elicitation using 5-azacytidine, a DNA methyltransferase inhibitor and suberoylanilide hydroxamic acid, a histone deacetylase inhibitor resulted in an increase in the number of active strains. Bioassay-guided isolation and structural elucidation yielded pestahivin and two new analogues from Bartalinia sp. F9447. Pestahivin and its related analogues did not exhibit antibacterial activity against Staphylococcus aureus but displayed strong antifungal activities against Candida albicans and Aspergillus brasiliensis, with IC50 values ranging from 0.46 ± 0.06 to 144 ± 18 µM. Pestahivin and its two analogues furthermore exhibited cytotoxic activity against A549 and MIA PACA-2 cancer cell lines with IC50 values in the range of 0.65 ± 0.12 to 42 ± 5.2 µM. The finding from this study reinforces that chemical epigenetic induction is a promising approach for the discovery of bioactive fungal secondary metabolites encoded by cryptic gene clusters.
ISSN: 2296-2646
DOI: 10.3389/fchem.2022.1024854
Schools: School of Biological Sciences 
Rights: © 2022 Munusamy, Ching, Yang, Crasta, Gakuubi, Chee, Wibowo, Leong, Kanagasundaram and Ng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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