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Title: Monitoring wound healing with topically applied optical NanoFlare mRNA nanosensors
Authors: Hwang, Jangsun
Seo, Youngmin
Jeong, Daun
Ning, Xiaoyu
Wiraja, Christian
Yang, Lixia
Tan, Chew Teng
Lee, Jinhyuck
Kim, Yesol
Kim, Ji Won
Kim, Dai Hyun
Choi, Jonghoon
Lim, Chin Yan
Pu, Kanyi
Jang, Woo Young
Xu, Chenjie
Keywords: Engineering::Bioengineering
Issue Date: 2022
Source: Hwang, J., Seo, Y., Jeong, D., Ning, X., Wiraja, C., Yang, L., Tan, C. T., Lee, J., Kim, Y., Kim, J. W., Kim, D. H., Choi, J., Lim, C. Y., Pu, K., Jang, W. Y. & Xu, C. (2022). Monitoring wound healing with topically applied optical NanoFlare mRNA nanosensors. Advanced Science, 9(18), e2104835-.
Project: A18A8b0059 
Journal: Advanced Science 
Abstract: An effective wound management strategy needs accurate assessment of wound status throughout the whole healing process. This can be achieved by examining molecular biomarkers including proteins, DNAs, and RNAs. However, existing methods for quantifying these biomarkers such as immunohistochemistry and quantitative polymerase chain reaction are usually laborious, resource-intensive, and disruptive. This article reports the development and utilization of mRNA nanosensors (i.e., NanoFlare) that are topically applied on cutaneous wounds to reveal the healing status through targeted and semi-quantitative examination of the mRNA biomarkers in skin cells. In 2D and 3D in vitro models, the efficacy and efficiency of these nanosensors are demonstrated in revealing the dynamic changes of mRNA biomarkers for different stages of wound development. In mouse models, this platform permits the tracking and identification of wound healing stages and a normal and diabetic wound healing process by wound healing index in real time.
ISSN: 2198-3844
DOI: 10.1002/advs.202104835
Schools: School of Chemical and Biomedical Engineering 
Interdisciplinary Graduate School (IGS) 
Research Centres: NTU Institute for Health Technologies
Rights: © 2022 The Authors. Advanced Science published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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