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dc.contributor.authorZhang, Hongyuen_US
dc.contributor.authorHuang, Zhuoyaen_US
dc.contributor.authorLv, Liuliuen_US
dc.contributor.authorXin, Yuyeen_US
dc.contributor.authorWang, Qianen_US
dc.contributor.authorLi, Fengen_US
dc.contributor.authorDong, Linaen_US
dc.contributor.authorWu, Changxinen_US
dc.contributor.authorIngham, Philip Williamen_US
dc.contributor.authorZhao, Zhonghuaen_US
dc.identifier.citationZhang, H., Huang, Z., Lv, L., Xin, Y., Wang, Q., Li, F., Dong, L., Wu, C., Ingham, P. W. & Zhao, Z. (2022). A transgenic zebrafish for in vivo visualization of cilia. Open Biology, 12(8), 220104-.
dc.description.abstractCilia are organelles for cellular signalling and motility. Mutations affecting ciliary function are also associated with cilia-related disorders (ciliopathies). The identification of cilia markers is critical for studying their function at the cellular level. Due to the lack of a conserved, short ciliary localization motif, the full-length ARL13b or 5HT6 proteins are normally used for cilia labelling. Overexpression of these genes, however, can affect the function of cilia, leading to artefacts in cilia studies. Here, we show that Nephrocystin-3 (Nphp3) is highly conserved among vertebrates and demonstrate that the N-terminal truncated peptide of zebrafish Nphp3 can be used as a gratuitous cilia-specific marker. To visualize the dynamics of cilia in vivo, we generated a stable transgenic zebrafish Tg (β-actin: nphp3N-mCherry)sx1001. The cilia in multiple cell types are efficiently labelled by the encoded fusion protein from embryonic stages to adulthood, without any developmental and physiological defects. We show that the line allows live imaging of ciliary dynamics and trafficking of cilia proteins, such as Kif7 and Smo, key regulators of the Hedgehog signalling pathway. Thus, we have generated an effective new tool for in vivo cilia studies that will help shed further light on the roles of these important organelles.en_US
dc.relation.ispartofOpen Biologyen_US
dc.rights© 2022 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License, which permits unrestricted use, provided the original author and source are credited.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleA transgenic zebrafish for in vivo visualization of ciliaen_US
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.description.versionPublished versionen_US
dc.subject.keywordsTransgenic Zebrafishen_US
dc.description.acknowledgementThis work was supported by National Natural Science Foundation of China (No. 31970738), Social Development Foundation of Shanxi (No. 201903D321019), Youth Foundation of Hundred Talents of Shanxi Province, and Fund Program for the Scientific Activities of Selected Returned Oversea Professionals in Shanxi Province, China.en_US
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