Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/166273
Title: A dual-locked tandem fluorescent probe for imaging of pyroptosis in cancer chemo-immunotherapy
Authors: Wang, Xinzhu
He, Shasha
Cheng, Penghui
Pu, Kanyi
Keywords: Science::Chemistry
Issue Date: 2023
Source: Wang, X., He, S., Cheng, P. & Pu, K. (2023). A dual-locked tandem fluorescent probe for imaging of pyroptosis in cancer chemo-immunotherapy. Advanced Materials, 35(10), 2206510-. https://dx.doi.org/10.1002/adma.202206510
Project: NRF-NRFI07-2021-0005 
2019-T1-002-045 
RG125/19 
RT05/20 
MOE-T2EP30220-0010 
MOE-T2EP30221-0004 
Journal: Advanced Materials 
Abstract: Real-time imaging of programmed cancer cell death (PCD) is imperative to monitor cancer therapeutic efficacy and tailor therapeutic regimens; however, specific in vivo detection of intratumoral pyroptosis remains challenging. Herein, a dual-locked and tandem activatable probe (DTAP) is reported for near-infrared fluorescence (NIRF) imaging of intratumoral pyroptosis during cancer chemo-immunotherapy in living mice. The probe comprises a hemicyanine dye dual-locked with an enzyme-responsive moiety that can be tandemly cleaved by pyroptosis-related biomarker (Caspase-1) and cancer biomarker (GGT) to turn on its NIRF signal. As pyroptosis plays a vital role in triggering anti-tumor immune responses, the activated signal of DTAP correlates well with the population of tumor-infiltrating cytotoxic T lymphocytes and tumor growth inhibition, consequently permitting the prediction of cancer therapeutic efficacy. This study also provides a non-invasive technique to study the regulatory mechanism of pyroptosis in cancer therapy and to optimize cancer chemo-immunotherapies for precision medicine.
URI: https://hdl.handle.net/10356/166273
ISSN: 0935-9648
DOI: 10.1002/adma.202206510
Schools: Lee Kong Chian School of Medicine (LKCMedicine) 
School of Chemistry, Chemical Engineering and Biotechnology 
Rights: © 2023 Wiley-VCH GmbH. All rights reserved. This is the peer reviewed version of the following article: Wang, X., He, S., Cheng, P. & Pu, K. (2023). A dual-locked tandem fluorescent probe for imaging of pyroptosis in cancer chemo-immunotherapy. Advanced Materials, 35(10), 2206510-, which has been published in final form at https://doi.org/10.1002/adma.202206510. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:CCEB Journal Articles
LKCMedicine Journal Articles

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