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Title: | Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins | Authors: | Koh, Jing Han | Keywords: | Science::Biological sciences | Issue Date: | 2023 | Publisher: | Nanyang Technological University | Source: | Koh, J. H. (2023). Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/167170 | Project: | MOE 2019-T3-1-012 | Abstract: | Nature has long been a source of inspiration for the design of advanced biomaterials with exceptional properties. One such inspiration is the Club Mineralization Protein-1, that plays a crucial role in the biomineralization of the mantis shrimps’ dactyl clubs. CMP-113, a truncated fusion protein inspired by the CMP-1, was designed to incorporate liquid-liquid phase separation (LLPS)-promoting motifs to investigate how phase separation can potentially contribute to biomineralization. In the presence of various Hofmeister salts, coacervation of CMP-113 was assessed through brightfield microscopy, turbidity assays, dynamic light scattering (DLS) measurements and nuclear magnetic resonance (NMR) spectroscopy. Its resistance to 1,6-hexanediol treatment and re-entrant LLPS in presence of calcium chloride suggests that LLPS is not purely hydrophobic-driven but may potentially electrostatic interactions as well. Morphological observations of crystals formed in biomineralization experiments not only demonstrated CMP-113’s capability to form hydroxyapatite from solution, but its coacervation may also be imperative for accelerating crystal nucleation and growth. These findings were consistent with the wider literature on non-classical nucleation pathways of biomineralization. Future research on CMP-113 and its applications in biomaterials and materials science is anticipated to unveil new insights and open up exciting opportunities in the field of biomaterials research. | URI: | https://hdl.handle.net/10356/167170 | Schools: | School of Biological Sciences | Fulltext Permission: | embargo_restricted_20250511 | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Student Reports (FYP/IA/PA/PI) |
Files in This Item:
File | Description | Size | Format | |
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Koh Jing Han_FYP Thesis FINAL.pdf Until 2025-05-11 | 2.62 MB | Adobe PDF | Under embargo until May 11, 2025 |
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