Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/16856
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dc.contributor.authorTan, Li Hua.
dc.date.accessioned2009-05-28T07:35:23Z
dc.date.available2009-05-28T07:35:23Z
dc.date.copyright2009en_US
dc.date.issued2009
dc.identifier.urihttp://hdl.handle.net/10356/16856
dc.description.abstractMalaria infection has been ongoing for the last 50,000 years, yet unlike other organisms, vaccine is still under-development. Experimental immunization with whole parasites live, physically or genetically attenuated provides successful protection in mice and humans. In order to identify the antigens involved in efficient immune response, we have developed tools and an assay to aid in the process of identification. Malaria antigens from p.yoelii and p.falciparum were cloned and expressed in mammalian cell surface display system. Using flow cytometry, nontransfected and transfected cells were characterized by labeling with anti-myc and the malaria fusion proteins were tested with anti-malaria antibodies. Similar transfection rates were observed in the transfected HEK293 and HEK293T cells but under drug pressure, only HEK293 cells could be used for positive transfectants selection. Culturing of plasmid containing cells in 200μg/ml geneticin increased percentage of cells containing plasmids. Transfected cells were specifically labeled with anti-malaria antibodies, proving reliability of the assay for use in screening for antigens recognized in immunized mice or humans.en_US
dc.format.extent39 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Science::Biological sciences::Microbiology::Immunologyen_US
dc.titleConstruction and validation of Plasmodium antigens surface display library in mammalian cells.en_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeBachelor of Science in Biological Sciencesen_US
dc.contributor.organizationA*STAR Singapore Immunology Networken_US
dc.contributor.supervisor2Laurent Reniaen_US
dc.contributor.supervisor2Gruner, Anne Charlotteen_US
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Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)
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