Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/169014
Title: Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation
Authors: Zhang, Liang 
Wang, Xiuping
Chen, Yun
Zhang, Baorui
Xu, Hui
Li, Chenchen
Zhou, Yan
Keywords: Engineering::Environmental engineering
Issue Date: 2023
Source: Zhang, L., Wang, X., Chen, Y., Zhang, B., Xu, H., Li, C. & Zhou, Y. (2023). Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation. Bioresource Technology, 374, 128805-. https://dx.doi.org/10.1016/j.biortech.2023.128805
Journal: Bioresource Technology 
Abstract: In this study, medium-chain fatty acid (MCFA) generation from mixed sludge (including primary sludge and waste activated sludge) was investigated without additional electron donors (EDs). 0.5 g COD/L of MCFAs was produced and the in situ generated ethanol could serve as the EDs during the anaerobic fermentation of mixed sludge without thermal hydrolysis process (THP) pretreatment. THP increased the MCFA production by approximately 128% in the anaerobic fermentation. During 102 days of operation, the fermentation of THP pre-treated mixed sludge stably generated 2.9 g COD/L MCFAs. The self-generated EDs could not maximize MCFA production, and external addition of ethanol improved MCFA yield. Caproiciproducens was the dominant chain-elongating bacteria. PICRUST2 revealed that both fatty acid biosynthesis and reverse β-oxidation pathways could participate in MCFA synthesis, and ethanol addition could enhance the contribution of the reverse β-oxidation pathway. Future studies should focus on the improvement of MCFA production from THP-assisted sludge fermentation.
URI: https://hdl.handle.net/10356/169014
ISSN: 0960-8524
DOI: 10.1016/j.biortech.2023.128805
Schools: School of Civil and Environmental Engineering 
Research Centres: Advanced Environmental Biotechnology Centre (AEBC) 
Nanyang Environment and Water Research Institute 
Rights: © 2023 Elsevier Ltd. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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