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Title: Investigation and screening of mixed microalgae species for lipase production and recovery using liquid biphasic flotation approach
Authors: Tang, Dories Ying Ying
Chu, Dinh-Toi
Nhi-Cong, Le Thi
Ratchahat, Sakhon
Banat, Fawzi
Chew, Kit Wayne
Gentili Francesco G.
Show, Pau Loke
Keywords: Engineering::Chemical engineering
Issue Date: 2023
Source: Tang, D. Y. Y., Chu, D., Nhi-Cong, L. T., Ratchahat, S., Banat, F., Chew, K. W., Gentili Francesco G. & Show, P. L. (2023). Investigation and screening of mixed microalgae species for lipase production and recovery using liquid biphasic flotation approach. Journal of the Taiwan Institute of Chemical Engineers, 142, 104646-.
Journal: Journal of the Taiwan Institute of Chemical Engineers
Abstract: Background: Lipase is mainly extracted from animals, plants, fungi, and bacteria. The difficulty in extracting lipase from these organisms has led to the discovery of sustainable sources producing lipase. This study is the first to document the existence of lipase in the microalgae species from Umeå. Methods: Lipase in microalgae was extracted using liquid biphasic flotation as the protein homogenate from the extracts was found to contain lipase activity. The lipase activity was evaluated using colorimetric method with p-nitrophenyl palmitate as the substrate. The types, volumes, and concentrations of solvents as well as flotation kinetics, and the biomass weight were optimized. The experiment was conducted at optimal temperature and pH 7. Significant Findings: The optimized conditions for maximum lipase production were 400 mg of biomass, 10 min flotation time with flotation rate of 100 cc/min as well as 99.8% ethanol and 300 g/L ammonium sulphate concentration with volume ratio of 1: 0.83. Lipase recovery yield of the optimal LBF system was 70.3% with the separation efficiency of 82.0% and purification factor of 7.45. The findings revealed that the microalgae can be an ideal candidate for producing lipase and LBF serves as the platform for the development of large-scale extraction system.
ISSN: 1876-1070
DOI: 10.1016/j.jtice.2022.104646
Schools: School of Chemistry, Chemical Engineering and Biotechnology 
Rights: © 2022 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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