Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/169058
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dc.contributor.authorLee, Jaslyn Jie Linen_US
dc.contributor.authorNg, Kuan Reien_US
dc.contributor.authorLiang, Jiaqien_US
dc.contributor.authorCui, Xien_US
dc.contributor.authorLi, Aaronen_US
dc.contributor.authorChen, Wei Ningen_US
dc.date.accessioned2023-06-27T08:52:51Z-
dc.date.available2023-06-27T08:52:51Z-
dc.date.issued2023-
dc.identifier.citationLee, J. J. L., Ng, K. R., Liang, J., Cui, X., Li, A. & Chen, W. N. (2023). Engineering the Phenylpropanoid pathway in Rhodosporidium toruloides for naringenin production from tyrosine by leveraging on its native PAL gene. ACS Food Science & Technology, 3(1), 92-99. https://dx.doi.org/10.1021/acsfoodscitech.2c00301en_US
dc.identifier.issn2692-1944en_US
dc.identifier.urihttps://hdl.handle.net/10356/169058-
dc.description.abstractFlavonoids are valued for their beneficial properties. This was a proof-of-concept study to show that the PAL gene in Rhodosporidium toruloides (R. toruloides) could be harnessed and only two genes 4CL and CHS needed to be inserted to create the naringenin producing strain, CBS-N. The growth of CBS-N reached a higher OD600 86.1 compared to 31.8 by wildtype. High-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) confirmed CBS-N successfully produced 0.038 mg/L naringenin and 16.9 mg/L p-coumaric acid via its PAL/TAL activity when supplemented with tyrosine, although the conversion efficiency of 4CL and CHS genes was the limiting factor. Metabolic profiling by gas chromatography-mass spectrometry (GC-MS) showed that, in the CBS-N strain, metabolic flux was toward glycolysis and the TCA cycle to produce malonyl CoA, the precursor for naringenin synthesis, and away from fatty acids synthesis pathway. Our study showed that microorganisms containing a native PAL gene, such as R. toruloides, could be harnessed for flavonoids production via precision fermentation.en_US
dc.language.isoenen_US
dc.relation.ispartofACS Food Science & Technologyen_US
dc.rights© 2022 American Chemical Society. All rights reserved.en_US
dc.subjectScience::Chemistryen_US
dc.titleEngineering the Phenylpropanoid pathway in Rhodosporidium toruloides for naringenin production from tyrosine by leveraging on its native PAL geneen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemistry, Chemical Engineering and Biotechnologyen_US
dc.identifier.doi10.1021/acsfoodscitech.2c00301-
dc.identifier.scopus2-s2.0-85144444833-
dc.identifier.issue1en_US
dc.identifier.volume3en_US
dc.identifier.spage92en_US
dc.identifier.epage99en_US
dc.subject.keywordsFlavonoidsen_US
dc.subject.keywordsNaringeninen_US
item.fulltextNo Fulltext-
item.grantfulltextnone-
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