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dc.contributor.authorJulca, Ireneen_US
dc.contributor.authorMutwil-Anderwald, Danielaen_US
dc.contributor.authorManoj, Vaishnervien_US
dc.contributor.authorKhan, Zahraen_US
dc.contributor.authorLai, Soak Kuanen_US
dc.contributor.authorYang, Lay K.en_US
dc.contributor.authorBeh, Ing Tsyren_US
dc.contributor.authorDziekan, Jerzyen_US
dc.contributor.authorLim, Yoon P.en_US
dc.contributor.authorLim, Shen Kiaten_US
dc.contributor.authorLow, Yee W.en_US
dc.contributor.authorLam, Yuen Inen_US
dc.contributor.authorTjia, Sethen_US
dc.contributor.authorMu, Yuguangen_US
dc.contributor.authorTan, Qiao Wenen_US
dc.contributor.authorNuc, Przemyslawen_US
dc.contributor.authorChoo, Le M.en_US
dc.contributor.authorKhew, Gillianen_US
dc.contributor.authorShining, Looen_US
dc.contributor.authorKam, Antonyen_US
dc.contributor.authorTam, James P.en_US
dc.contributor.authorBozdech, Zbyneken_US
dc.contributor.authorSchmidt, Maximilianen_US
dc.contributor.authorUsadel, Bjoernen_US
dc.contributor.authorKanagasundaram, Yoganathanen_US
dc.contributor.authorAlseekh, Salehen_US
dc.contributor.authorFernie, Alisdairen_US
dc.contributor.authorLi, Hoi Y.en_US
dc.contributor.authorMutwil, Mareken_US
dc.identifier.citationJulca, I., Mutwil-Anderwald, D., Manoj, V., Khan, Z., Lai, S. K., Yang, L. K., Beh, I. T., Dziekan, J., Lim, Y. P., Lim, S. K., Low, Y. W., Lam, Y. I., Tjia, S., Mu, Y., Tan, Q. W., Nuc, P., Choo, L. M., Khew, G., Shining, L., ...Mutwil, M. (2023). Genomic, transcriptomic, and metabolomic analysis of Oldenlandia corymbosa reveals the biosynthesis and mode of action of anti-cancer metabolites. Journal of Integrative Plant Biology, 65(6), 1442-1466.
dc.description.abstractPlants accumulate a vast array of secondary metabolites, which constitute a natural resource for pharmaceuticals. Oldenlandia corymbosa belongs to the Rubiaceae family, and has been used in traditional medicine to treat different diseases, including cancer. However, the active metabolites of the plant, their biosynthetic pathway and mode of action in cancer are unknown. To fill these gaps, we exposed this plant to eight different stress conditions and combined different omics data capturing gene expression, metabolic profiles, and anti-cancer activity. Our results show that O. corymbosa extracts are active against breast cancer cell lines and that ursolic acid is responsible for this activity. Moreover, we assembled a high-quality genome and uncovered two genes involved in the biosynthesis of ursolic acid. Finally, we also revealed that ursolic acid causes mitotic catastrophe in cancer cells and identified three high-confidence protein binding targets by Cellular Thermal Shift Assay (CETSA) and reverse docking. Altogether, these results constitute a valuable resource to further characterize the biosynthesis of active metabolites in the Oldenlandia group, while the mode of action of ursolic acid will allow us to further develop this valuable compound.en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.relation.ispartofJournal of Integrative Plant Biologyen_US
dc.rights© 2023 The Authors. Journal of Integrative Plant Biology published by John Wiley & Sons Australia, Ltd on behalf of Institute of Botany, Chinese Academy of Sciences. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleGenomic, transcriptomic, and metabolomic analysis of Oldenlandia corymbosa reveals the biosynthesis and mode of action of anti-cancer metabolitesen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.organizationDepartment of Biochemistry, NUSen_US
dc.contributor.organizationSingapore Botanic Gardensen_US
dc.description.versionPublished versionen_US
dc.description.acknowledgementI.J. is supported by Nanyang Biologics, M.M. is supported by a NTU Start‐Up Grant and Singaporean Ministry of Education grant MOE2018‐T2‐2‐053. J.M.D (NTU‐PPF‐2019). Y.K. is supported by Natural Product Research Laboratory Biomedical Research Council of A*STAR (Agency for Science, Technology and Research) Transition Fund (H16/99/b0/004), the Singapore Institute of Food and Biotechnology Innovation core fund.en_US
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