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Title: Genomic, transcriptomic, and metabolomic analysis of Oldenlandia corymbosa reveals the biosynthesis and mode of action of anti-cancer metabolites
Authors: Julca, Irene
Mutwil-Anderwald, Daniela
Manoj, Vaishnervi
Khan, Zahra
Lai, Soak Kuan
Yang, Lay K.
Beh, Ing Tsyr
Dziekan, Jerzy
Lim, Yoon P.
Lim, Shen Kiat
Low, Yee W.
Lam, Yuen In
Tjia, Seth
Mu, Yuguang
Tan, Qiao Wen
Nuc, Przemyslaw
Choo, Le M.
Khew, Gillian
Shining, Loo
Kam, Antony
Tam, James P.
Bozdech, Zbynek
Schmidt, Maximilian
Usadel, Bjoern
Kanagasundaram, Yoganathan
Alseekh, Saleh
Fernie, Alisdair
Li, Hoi Y.
Mutwil, Marek
Keywords: Science::Biological sciences
Issue Date: 2023
Source: Julca, I., Mutwil-Anderwald, D., Manoj, V., Khan, Z., Lai, S. K., Yang, L. K., Beh, I. T., Dziekan, J., Lim, Y. P., Lim, S. K., Low, Y. W., Lam, Y. I., Tjia, S., Mu, Y., Tan, Q. W., Nuc, P., Choo, L. M., Khew, G., Shining, L., ...Mutwil, M. (2023). Genomic, transcriptomic, and metabolomic analysis of Oldenlandia corymbosa reveals the biosynthesis and mode of action of anti-cancer metabolites. Journal of Integrative Plant Biology, 65(6), 1442-1466.
Project: NTU-SUG 
Journal: Journal of Integrative Plant Biology 
Abstract: Plants accumulate a vast array of secondary metabolites, which constitute a natural resource for pharmaceuticals. Oldenlandia corymbosa belongs to the Rubiaceae family, and has been used in traditional medicine to treat different diseases, including cancer. However, the active metabolites of the plant, their biosynthetic pathway and mode of action in cancer are unknown. To fill these gaps, we exposed this plant to eight different stress conditions and combined different omics data capturing gene expression, metabolic profiles, and anti-cancer activity. Our results show that O. corymbosa extracts are active against breast cancer cell lines and that ursolic acid is responsible for this activity. Moreover, we assembled a high-quality genome and uncovered two genes involved in the biosynthesis of ursolic acid. Finally, we also revealed that ursolic acid causes mitotic catastrophe in cancer cells and identified three high-confidence protein binding targets by Cellular Thermal Shift Assay (CETSA) and reverse docking. Altogether, these results constitute a valuable resource to further characterize the biosynthesis of active metabolites in the Oldenlandia group, while the mode of action of ursolic acid will allow us to further develop this valuable compound.
ISSN: 1672-9072
DOI: 10.1111/jipb.13469
Schools: School of Biological Sciences 
Organisations: Department of Biochemistry, NUS 
Singapore Botanic Gardens 
Rights: © 2023 The Authors. Journal of Integrative Plant Biology published by John Wiley & Sons Australia, Ltd on behalf of Institute of Botany, Chinese Academy of Sciences. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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