Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/16992
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dc.contributor.authorGoh, Xuewei.-
dc.date.accessioned2009-05-29T03:12:52Z-
dc.date.available2009-05-29T03:12:52Z-
dc.date.copyright2009en_US
dc.date.issued2009-
dc.identifier.urihttp://hdl.handle.net/10356/16992-
dc.description.abstractRUNX2 is a key regulator of bone development and it controls genes that contribute to cell growth regulation in osteoblast. This study investigated the role that RUNX2 play in the cell proliferation regulatory pathways of breast cancer cells. From our serum stimulation study of MDA-MB-231 cells, results showed that RUNX2 protein levels decreased and proliferation of breast cancer cells was enhanced. Our quantitative RT-PCR results showed approximately 5-fold higher levels of cyclin D1 in MDA-MB-231 cells with knockdown of RUNX2; further suggest the involvement of RUNX2 in inhibiting cell proliferation. ERK pathway is the best characterized MAPK pathway in mammalian cells, which plays a key role in cell proliferation. From our study, we showed that ERK pathway is also involved in the upstream regulation of RUNX2 in breast cancer cells. MDA-MB-231 cells were treated with MEK1 inhibitor PD98059 and results showed a gradual decrease in ERK1/2 phosphorylation and also a modest increase in RUNX2 protein level. From this study, we showed an inverse correlation between ERK1/2 phosphorylation and RUNX2 protein level. In conclusion this study suggested that RUNX2 may inhibit cell proliferation in breast cancer cells and its level is regulated by ERK pathway.en_US
dc.format.extent33 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University-
dc.subjectDRNTU::Science::Biological sciences::Cytologyen_US
dc.titleRole of RUNX2 in mitogen-activated protein kinase (MAPK) mediated cell proliferation of breast cancer cells.en_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorTa Nguyen Binh Duongen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeBachelor of Science in Biological Sciencesen_US
dc.contributor.organizationOncology Research Instituteen_US
dc.contributor.supervisor2Leong, David Tai Weien_US
dc.contributor.supervisor2Manuel Salto-Tellezen_US
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Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)
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