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dc.contributor.authorSamandari, Tarazen_US
dc.contributor.authorOngalo, Joshua B.en_US
dc.contributor.authorMcCarthy, Kimberly D.en_US
dc.contributor.authorBiegon, Richard K.en_US
dc.contributor.authorMadiega, Philister A.en_US
dc.contributor.authorMithika, Anneen_US
dc.contributor.authorOrinda, Josephen_US
dc.contributor.authorMboya, Grace M.en_US
dc.contributor.authorMwaura, Patricken_US
dc.contributor.authorAnzala, Omuen_US
dc.contributor.authorOnyango, Claytonen_US
dc.contributor.authorOluoch, Fredrick O.en_US
dc.contributor.authorOsoro, Ericen_US
dc.contributor.authorDutertre, Charles-Antoineen_US
dc.contributor.authorTan, Nicoleen_US
dc.contributor.authorHang, Shou Kiten_US
dc.contributor.authorHariharaputran, Smrithien_US
dc.contributor.authorLye, David C.en_US
dc.contributor.authorHerman-Roloff, Amyen_US
dc.contributor.authorLe Bert, Ninaen_US
dc.contributor.authorBertoletti, Antonioen_US
dc.identifier.citationSamandari, T., Ongalo, J. B., McCarthy, K. D., Biegon, R. K., Madiega, P. A., Mithika, A., Orinda, J., Mboya, G. M., Mwaura, P., Anzala, O., Onyango, C., Oluoch, F. O., Osoro, E., Dutertre, C., Tan, N., Hang, S. K., Hariharaputran, S., Lye, D. C., Herman-Roloff, A., ...Bertoletti, A. (2023). Prevalence and functional profile of SARS-CoV-2 T cells in asymptomatic Kenyan adults. Journal of Clinical Investigation, 133(13), e170011-.
dc.description.abstractBackground: SARS-CoV-2 infection in Africa has been characterized by a less severe disease profile than what has been observed elsewhere, but the profile of SARS-CoV-2-specific adaptive immunity in these mainly asymptomatic patients has not, to our knowledge, been analyzed. Methods: We collected blood samples from residents of rural Kenya (n = 80), who had not experienced any respiratory symptoms or had contact with individuals with COVID-19 and had not received COVID-19 vaccines. We analyzed spike-specific antibodies and T cells specific for SARS-CoV-2 structural (membrane, nucleocapsid, and spike) and accessory (ORF3a, ORF7, ORF8) proteins. Pre-pandemic blood samples collected in Nairobi (n = 13) and blood samples from mild-to-moderately symptomatic COVID-19 convalescent patients (n = 36) living in the urban environment of Singapore were also studied. Results: Among asymptomatic Africans, we detected anti-spike antibodies in 41.0% of the samples and T cell responses against 2 or more SARS-CoV-2 proteins in 82.5% of samples examined. Such a pattern was absent in the pre-pandemic samples. Furthermore, distinct from cellular immunity in European and Asian COVID-19 convalescents, we observed strong T cell immunogenicity against viral accessory proteins (ORF3a, ORF8) but not structural proteins, as well as a higher IL-10/IFN-γ cytokine ratio profile. Conclusions: The high incidence of T cell responses against different SARS-CoV-2 proteins in seronegative participants suggests that serosurveys underestimate SARS-CoV-2 prevalence in settings where asymptomatic infections prevail. The functional and antigen-specific profile of SARS-CoV-2-specific T cells in African individuals suggests that environmental factors can play a role in the development of protective antiviral immunity.en_US
dc.description.sponsorshipMinistry of Health (MOH)en_US
dc.description.sponsorshipNational Medical Research Council (NMRC)en_US
dc.relation.ispartofJournal of Clinical Investigationen_US
dc.rights© 2023 Samandari et al. This is an open access article published under the terms of the Creative Commons Attribution 4.0 International License.en_US
dc.titlePrevalence and functional profile of SARS-CoV-2 T cells in asymptomatic Kenyan adultsen_US
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.contributor.organizationNational Centre for Infectious Diseases, Singaporeen_US
dc.contributor.organizationTan Tock Seng Hospitalen_US
dc.contributor.organizationYong Loo Lin School of Medicine, NUSen_US
dc.description.versionPublished versionen_US
dc.subject.keywordsAsymptomatic Coronavirus Disease 2019en_US
dc.description.acknowledgementFunding for this research was provided by the US Centers for Disease Control and Prevention, Division of Global Health Protection, the Singapore Ministry of Health’s National Medical Research Council under its COVID-19 Research Fund (COVID19RF3-0060, COVID19RF-001 and COVID19RF-008), and the Singapore Ministry of Health’s National Medical Research Council MOH-000019 (MOH-StaR17Nov-0001).en_US
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