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Title: Structural study of G-rich sequences: G4, non-G4 and G-wires
Authors: Tan, Keith Wei Rong
Keywords: Science::Biological sciences::Biophysics
Issue Date: 2023
Publisher: Nanyang Technological University
Source: Tan, K. W. R. (2023). Structural study of G-rich sequences: G4, non-G4 and G-wires. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: The study of nucleic acids is essential in understanding genetics as they are the very foundation of life as we know it. In our study, we aim to uncover more about the structure and interactions of Guanine-rich nucleic acids. We look at them with different means, mainly by nuclear magnetic resonance (NMR), ultraviolet absorption (UV) spectroscopy, circular dichroism spectroscopy (CD) and gel electrophoresis. With the spectroscopic techniques, we break the original assumption that only G-quadruplexes form in a G-quadruplex forming sequence by showing the presence of an unreported species, the non-G4 species. Lastly, with the aid of gel electrophoresis, we demonstrate that G-wires, another structure that can be formed by G-rich sequences are more stable than G-quadruplexes.
DOI: 10.32657/10356/171776
Schools: School of Physical and Mathematical Sciences 
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
Fulltext Permission: embargo_20251108
Fulltext Availability: With Fulltext
Appears in Collections:SPMS Theses

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