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dc.contributor.authorSu, Wan-Chihen_US
dc.contributor.authorHo, James Chin Shingen_US
dc.contributor.authorGettel, Douglas L.en_US
dc.contributor.authorRowland, Andrew T.en_US
dc.contributor.authorKeating, Christine D.en_US
dc.contributor.authorParikh, Atul N.en_US
dc.identifier.citationSu, W., Ho, J. C. S., Gettel, D. L., Rowland, A. T., Keating, C. D. & Parikh, A. N. (2023). Kinetic control of shape deformations and membrane phase separation inside giant vesicles. Nature Chemistry.
dc.description.abstractA variety of cellular processes use liquid-liquid phase separation (LLPS) to create functional levels of organization, but the kinetic pathways by which it proceeds remain incompletely understood. Here in real time, we monitor the dynamics of LLPS of mixtures of segregatively phase-separating polymers inside all-synthetic, giant unilamellar vesicles. After dynamically triggering phase separation, we find that the ensuing relaxation-en route to the new equilibrium-is non-trivially modulated by a dynamic interplay between the coarsening of the evolving droplet phase and the interactive membrane boundary. The membrane boundary is preferentially wetted by one of the incipient phases, dynamically arresting the progression of coarsening and deforming the membrane. When the vesicles are composed of phase-separating mixtures of common lipids, LLPS within the vesicular interior becomes coupled to the membrane's compositional degrees of freedom, producing microphase-separated membrane textures. This coupling of bulk and surface phase-separation processes suggests a physical principle by which LLPS inside living cells might be dynamically regulated and communicated to the cellular boundaries.en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.relation.ispartofNature Chemistryen_US
dc.rights© 2023 The Author(s), under exclusive licence to Springer Nature Limited. All rights reserveden_US
dc.subjectEngineering::Chemical engineeringen_US
dc.titleKinetic control of shape deformations and membrane phase separation inside giant vesiclesen_US
dc.typeJournal Articleen
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineeringen_US
dc.contributor.researchInstitute For Digital Molecular Analytics and Scienceen_US
dc.subject.keywordsSpinodal Decompositionen_US
dc.subject.keywordsDomain Growthen_US
dc.description.acknowledgementW.-C.S., D.L.G. and A.N.P. acknowledge funding from the National Science Foundation (DMR-1810540). J.C.S.H. and A.N.P. acknowledge funding and support from the Singapore Centre for Environmental Life Sciences Engineering and the Institute for Digital Molecular Analytics and Science, Nanyang Technological University. C.D.K. and A.T.R. were supported by the US Department of Energy, Office of Science, Basic Energy Sciences under award no. DE-SC0008633.en_US
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