Epithelial tissue wound healing process on 3D substrate

Abstract

Wound healing is a complex biomechanical process sensitive to numerous physical, chemical, and environmental factors. The wound healing process has been extensively investigate using two-dimensional (2D) substrates. However, it remains unclear how substrate topography may affect the wound healing process. This project’s goal is to understand and analyze the wound healing process of epithelial tissues on 3D substrates with micron-sized wells. In this dissertation, we dive into the most minute details of factors in varying environment such as manipulation in diameter of wound wells to understand the cell growth process well. Besides that, this paper explores the impact of gravity on the cell growth of epithelial cells on the 3D substrates to understand how these variables may potentially impact the wound healing rate which has the potential to contribute to the wound healing of epithelial tissues.