Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/175591
Title: PICLS with human cells is the first high throughput screening method for identifying novel compounds that extend lifespan
Authors: Alfatah, Mohammad
Zhang, Yizhong
Naaz, Arshia
Cheng, Trishia Yi Ning
Eisenhaber, Frank
Keywords: Medicine, Health and Life Sciences
Issue Date: 2024
Source: Alfatah, M., Zhang, Y., Naaz, A., Cheng, T. Y. N. & Eisenhaber, F. (2024). PICLS with human cells is the first high throughput screening method for identifying novel compounds that extend lifespan. Biology Direct, 19(1), 8-. https://dx.doi.org/10.1186/s13062-024-00455-4
Project: C210112008 
MOH-001348-00 
Journal: Biology Direct 
Abstract: Gerontology research on anti-aging interventions with drugs could be an answer to age-related diseases, aiming at closing the gap between lifespan and healthspan. Here, we present two methods for assaying chronological lifespan in human cells: (1) a version of the classical outgrowth assay with quantitative assessment of surviving cells and (2) a version of the PICLS method (propidium iodide fluorescent-based measurement of cell death). Both methods are fast, simple to conduct, cost-effective, produce quantitative data for further analysis and can be used with diverse human cell lines. Whereas the first method is ideal for validation and testing the post-intervention reproductive potential of surviving cells, the second method has true high-throughput screening potential. The new technologies were validated with known anti-aging compounds (2,5-anhydro-D-mannitol and rapamycin). Using the high-throughput screening method, we screened a library of 162 chemical entities and identified three compounds that extend the longevity of human cells.
URI: https://hdl.handle.net/10356/175591
ISSN: 1745-6150
DOI: 10.1186/s13062-024-00455-4
Schools: School of Biological Sciences 
Organisations: Bioinformatics Institute, A*STAR 
Rights: © The Author(s) 2024. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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