Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/180031
Title: Using an ER-specific optogenetic mechanostimulator to understand the mechanosensitivity of the endoplasmic reticulum
Authors: Song, Yutong
Zhao, Zhihao
Xu, Linyu
Huang, Peiyuan
Gao, Jiayang
Li, Jingxuan
Wang, Xuejie
Zhou, Yiren
Wang, Jinhui
Zhao, Wenting
Wang, Likun
Zheng, Chaogu
Gao, Bo
Jiang, Liwen
Liu, Kai
Guo, Yusong
Yao, Xiaoqiang
Duan, Liting
Keywords: Medicine, Health and Life Sciences
Issue Date: 2024
Source: Song, Y., Zhao, Z., Xu, L., Huang, P., Gao, J., Li, J., Wang, X., Zhou, Y., Wang, J., Zhao, W., Wang, L., Zheng, C., Gao, B., Jiang, L., Liu, K., Guo, Y., Yao, X. & Duan, L. (2024). Using an ER-specific optogenetic mechanostimulator to understand the mechanosensitivity of the endoplasmic reticulum. Developmental Cell, 59(11), 1396-1409.e5. https://dx.doi.org/10.1016/j.devcel.2024.03.014
Journal: Developmental Cell
Abstract: The ability of cells to perceive and respond to mechanical cues is essential for numerous biological activities. Emerging evidence indicates important contributions of organelles to cellular mechanosensitivity and mechanotransduction. However, whether and how the endoplasmic reticulum (ER) senses and reacts to mechanical forces remains elusive. To fill the knowledge gap, after developing a light-inducible ER-specific mechanostimulator (LIMER), we identify that mechanostimulation of ER elicits a transient, rapid efflux of Ca2+ from ER in monkey kidney COS-7 cells, which is dependent on the cation channels transient receptor potential cation channel, subfamily V, member 1 (TRPV1) and polycystin-2 (PKD2) in an additive manner. This ER Ca2+ release can be repeatedly stimulated and tuned by varying the intensity and duration of force application. Moreover, ER-specific mechanostimulation inhibits ER-to-Golgi trafficking. Sustained mechanostimuli increase the levels of binding-immunoglobulin protein (BiP) expression and phosphorylated eIF2α, two markers for ER stress. Our results provide direct evidence for ER mechanosensitivity and tight mechanoregulation of ER functions, placing ER as an important player on the intricate map of cellular mechanotransduction.
URI: https://hdl.handle.net/10356/180031
ISSN: 1534-5807
DOI: 10.1016/j.devcel.2024.03.014
Schools: School of Chemical and Biomedical Engineering 
Rights: © 2024 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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