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https://hdl.handle.net/10356/180031
Title: | Using an ER-specific optogenetic mechanostimulator to understand the mechanosensitivity of the endoplasmic reticulum | Authors: | Song, Yutong Zhao, Zhihao Xu, Linyu Huang, Peiyuan Gao, Jiayang Li, Jingxuan Wang, Xuejie Zhou, Yiren Wang, Jinhui Zhao, Wenting Wang, Likun Zheng, Chaogu Gao, Bo Jiang, Liwen Liu, Kai Guo, Yusong Yao, Xiaoqiang Duan, Liting |
Keywords: | Medicine, Health and Life Sciences | Issue Date: | 2024 | Source: | Song, Y., Zhao, Z., Xu, L., Huang, P., Gao, J., Li, J., Wang, X., Zhou, Y., Wang, J., Zhao, W., Wang, L., Zheng, C., Gao, B., Jiang, L., Liu, K., Guo, Y., Yao, X. & Duan, L. (2024). Using an ER-specific optogenetic mechanostimulator to understand the mechanosensitivity of the endoplasmic reticulum. Developmental Cell, 59(11), 1396-1409.e5. https://dx.doi.org/10.1016/j.devcel.2024.03.014 | Journal: | Developmental Cell | Abstract: | The ability of cells to perceive and respond to mechanical cues is essential for numerous biological activities. Emerging evidence indicates important contributions of organelles to cellular mechanosensitivity and mechanotransduction. However, whether and how the endoplasmic reticulum (ER) senses and reacts to mechanical forces remains elusive. To fill the knowledge gap, after developing a light-inducible ER-specific mechanostimulator (LIMER), we identify that mechanostimulation of ER elicits a transient, rapid efflux of Ca2+ from ER in monkey kidney COS-7 cells, which is dependent on the cation channels transient receptor potential cation channel, subfamily V, member 1 (TRPV1) and polycystin-2 (PKD2) in an additive manner. This ER Ca2+ release can be repeatedly stimulated and tuned by varying the intensity and duration of force application. Moreover, ER-specific mechanostimulation inhibits ER-to-Golgi trafficking. Sustained mechanostimuli increase the levels of binding-immunoglobulin protein (BiP) expression and phosphorylated eIF2α, two markers for ER stress. Our results provide direct evidence for ER mechanosensitivity and tight mechanoregulation of ER functions, placing ER as an important player on the intricate map of cellular mechanotransduction. | URI: | https://hdl.handle.net/10356/180031 | ISSN: | 1534-5807 | DOI: | 10.1016/j.devcel.2024.03.014 | Schools: | School of Chemical and Biomedical Engineering | Rights: | © 2024 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SCBE Journal Articles |
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