Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/18035
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dc.contributor.authorKo, Chen Wei.-
dc.date.accessioned2009-06-19T02:21:48Z-
dc.date.available2009-06-19T02:21:48Z-
dc.date.copyright2009en_US
dc.date.issued2009-
dc.identifier.urihttp://hdl.handle.net/10356/18035-
dc.description.abstractThe Human T-Lymphotropic virus type 1 (HTLV-1) genome codes for a 40 kD viral protein Tax which can trans activate the human cytomegalovirus (CMV) major immediate-early (MIE) enhancer in Jurkat T cells. The CMV MIE promoter is one of the strongest RNA polymerase II identified and is used industrially for recombinant protein production. It is envisaged that a Tax expressing cell line could be used to increase CMV MIE promoter activity for recombinant protein production. Tax was cloned into pcDNA6/myc-His with a selection marker blasticidin-resistance gene for the generation of Tax stable mammalian cell lines. The plasmid was expressed in Human Embroyonic Kidney 293 (HEK293) cells and induced a 10-fold and 50-fold increase in CMV-Luciferase (CMV-Luc) and HTLV-Long Terminal Repeat (LTR)-Luciferase (HTLV-LTR-Luc) activity respectively. The effects of Tax interacting transcription factors which may increase CMV MIE promoter activation were also investigated. 4 members of the cyclic-AMP-responsive element binding protein/activating transcription factors (CREB/ATF) family of transcription factors, namely the X-box binding protein 1 (XBP-1), its spliced form XBP-1S, CREB1 and CREB2 were overexpressed in HEK293 cells in the presence and absence of Tax. CREB1 was found to inhibit CMV MIE promoter activity by 2 fold.en_US
dc.format.extent37 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University-
dc.subjectDRNTU::Scienceen_US
dc.titleEffects of HTLV-1 tax and CREB/ATF family of transcription factors on CMV MIE promoter in HEK293 cells.en_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorChao Shu Ting Edlynen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeBachelor of Science in Biological Sciencesen_US
dc.contributor.supervisor2Chao Sheng-Haoen_US
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Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)
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