Nanoparticles modulating mesenchymal stem cells secretome for regenerative medicine

Abstract

Wound healing has been a challenge due to various factors such as infection, hypoxia and medical conditions. The use of nanomaterials have become more prominent with nanoparticles being used commonly for wide variety of medical treatments. Multiple variants of nanoparticles are currently in use with metallic nanoparticles being a common usage for medical applications. A variant known as Mesoporous Silica Nanoparticles (MSN) have been showing effectiveness in the use of drug delivery. This study seeks to determine the ability of MSN to induce oxidative stress to Mesenchymal Stem Cells (MSC) to stimulate secretion of angiogenic factors and to determine the use of conjugated amphibian-dervied peptides to promote the secretion of pro-angiogenic factors for wound healing. Nanoparticles was synthesised to achieve four different varients namely MSN, Amine functionalised MSN (MSN-NH2), MSN with Amine conjugated with Peptides (MSN-Peptide) and pure silica nanoparticles (NP Silica). Characterisation was conducted on the nanoparticles which confirms the presences of amine groups in MSN-NH2 and the nanoparticles size and zeta potential was charactereised. Conjugation of amphibian-dervied peptides was deemed successful to proceed and the degree of hydrolysis was also suggested to determine the increase in hydrodynamic diameter of MSN-Peptide. Evaluation on the cell viability on the optimal concentration for further analysis to determine the nanoparticles uptake and Reactive Oxygen Species (ROS) was conducted. Results shows MSN able to survive at high concentration of 200μg/mL with high ROS expression and nanoparticles uptake. These findings from wound healing assay suggest that MSN-Peptide and MSN have promising therapeutic effectiveness in closing a wound which suggest the potential usage of MSN and the amphibian-derived peptides. Although a possibility arise where amphibian-dervied peptide may provide a therapeutic effect without conjugation with nanoparticles, this serves as a foundation for further exploration to determine the full effectiveness on the use of MSN and MSN-Peptide in wound healing application.