Detection of variant of hepatitis B virus and beta-globin through DNA technology

Abstract

Two systems, i) the HBV precore gene, and ii) the human 0-globin gene were used as models to develop simple, reliable and fast methods for the detection of point mutations. Three PCR-based methods were employed to detect the HBV precore stop codon mutant. They are RG-PCR, SSCP analysis and non-radioactive direct DNA cycle sequencing.