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Title: Design and construction of optical tweezers : single-molecule study of DNA mechanical properties.
Authors: Lim, Ci Ji.
Keywords: DRNTU::Science::Biological sciences::Biophysics
Issue Date: 2009
Abstract: Optical tweezer is an integral tool in the research frontiers of science and engineering. In this FYP, a dual beam optical tweezers with single molecule detection capability is designed and constructed. The process of design and construction is discussed in this thesis. The constructed optical tweezer has a laser power throughput of 66% and maximum optical trapping force of 50 pN. Fundamental experiments were done to demonstrate the optical tweezer applicability. 3 um polystyrene beads coated with fluorescent-tagged complementary ssDNA were optically manipulated to form stable heterogenous pair. Preliminary optical pulling of a 1 um dsDNA tether was also done using the constructed optical tweezers.
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SPMS Student Reports (FYP/IA/PA/PI)

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