Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/20706
Title: Effects of different scan factors in genome analyser (illumina) platform on gene expression profiling
Authors: Kwok, Wee Chiew.
Keywords: DRNTU::Engineering::Bioengineering
Issue Date: 2009
Abstract: The DNA is the fundamental underlying principle responsible for differences between any two living organisms. However, understanding the 3 billion base pair human genome is highly arduous and requires sophisticated genomic analysis equipment such as the microarray. Illumina microarray, which uses advanced BeadArray Technology, is becoming increasingly popular due to its ability to produce more reliable results when compared to conventional microarray. Thus, Illumina microarray data is used for this study. Genomic studies necessitate three simple criteria: precision, consistency and completeness. Thus, Illumina microarray data used for genomic analysis must fulfill the criteria. One variable that affects the criteria is the Scan Factor that controls the amount of incident light on the microarray chip during sample scanning. In this project, the effects of different Scan Factor on the microarray data is studied using 3 defined statistical methods, S/N ratio, Dynamic Range and Illuminance intensity distribution. The report also provides a suggestion on incorporating different Scan Factors to improve the completeness and accuracy of the Illumina data.
URI: http://hdl.handle.net/10356/20706
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
KwokWeeChiew09.pdf
  Restricted Access
1.06 MBAdobe PDFView/Open

Page view(s)

347
Updated on Dec 4, 2020

Download(s)

3
Updated on Dec 4, 2020

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.