Functional analysis of mammalian homologues of yeast vrp1p using S. cerevisiae

Abstract

Cells are the basic units of life. The eukaryotic cells are composed of cell membrane, organelles, nucleus and cytoplasm. The majority of cellular metabolism takes place in the cytosol which consists of enzymes to carry out biochemical reactions as well as proteins with structural functions. Nearly half of the total proteins in a cell are located in the cytosol. Protein complexes are assembled and disassembled through out the cell cycle. If an eukaryotic cell is permeablized by nonionic detergent the soluble protein from the cytoplasm will diffuse out, leaving behind the cytoskeletal filaments. The cytoskeleton is highly organized and some of the cellular proteins rely on the cytoskeletal filaments for localization to specific regions within the cell.