Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/38869
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dc.contributor.authorTeo, Hutt Wang.
dc.date.accessioned2010-05-20T02:33:16Z
dc.date.available2010-05-20T02:33:16Z
dc.date.copyright2010en_US
dc.date.issued2010
dc.identifier.urihttp://hdl.handle.net/10356/38869
dc.description.abstractMalaria is a deadly disease that is responsible for 300-500 million infections and kills about 1 million people annually. As a vaccine is still not available, chloroquine has been the anchor in the treatment of malaria and is the preferred drug. However, with the spread of chloroquine resistance, the need for an alternative drug with comparable efficiency is pressing. Artemisinin derivatives have the same efficacy as chloroquine and are the recommended drug to treat severe cases of malaria, such as cerebral malaria (CM). Since the malaria parasites sequester in deep organs, we investigated whether these drugs inhibit with the same efficacy, sequestered parasites and peripheral parasites. Thus, we infected mice with Plasmodium parasites and used bioluminescent imaging to monitor parasite sequestration throughout treatment. Concurrently, peripheral blood parasitemia was monitored by flow cytometry. We found that 10-days treatment of both artesunate and chloroquine prevented the development of experimental cerebral malaria (ECM) in C57BL/6J mice and prolonged their survival. Furthermore, artesunate’s efficiency was higher in BALB/cJ mice compared to C57BL/6J mice, but the recrudescence rate was high. Chloroquine treatment eradicated the parasites completely without recrudescence. Thus, the results suggested that artesunate is not as effective as chloroquine in treating murine malaria, and a longer course and dosage of treatment is required to have the equivalent chloroquine effect.en_US
dc.format.extent31 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Science::Biological sciences::Microbiologyen_US
dc.titleIn vivo quantification of artesunate and chloroquine effect in the development of luciferase expressing murine Plasmodium parasites in C57BL/6 and BABL/c mice.en_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeBachelor of Science in Biological Sciencesen_US
dc.contributor.organizationA*STAR Singapore Immunology Networken_US
dc.contributor.supervisor2Laurent Reniaen_US
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Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)
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