dc.contributor.authorChen, Bo
dc.date.accessioned2010-05-21T06:41:54Z
dc.date.accessioned2017-07-23T08:42:16Z
dc.date.available2010-05-21T06:41:54Z
dc.date.available2017-07-23T08:42:16Z
dc.date.copyright2008en_US
dc.date.issued2008
dc.identifier.citationChen, B. (2008). Functional characterization of coronavirus non-structural proteins. Doctoral thesis, Nanyang Technological University, Singapore.
dc.identifier.urihttp://hdl.handle.net/10356/39373
dc.description.abstractGenetic manipulation of the RNA genomes by reverse genetics is a powerful tool to study the molecular biology and pathogenesis of RNA viruses. During construction of an infectious clone from a Vero cell-adapted coronavirus infectious bronchitis virus (IBV), we found that a G - C point mutation at nucleotide position 15526, causing Arg-to-Pro mutation at amino acid position 132 of the helicase protein, is lethal to the infectivity of IBV on Vero cells. When the in vitro-synthesized full-length transcripts containing this mutation were introduced into Vero cells, no infectious virus was rescued. Upon correction of the mutation, infectious virus was recovered. Further characterization of the in vitro-synthesized full-length transcripts containing the G15526C mutation demonstrated that this mutation may block the transcription of subgenomic RNAs.en_US
dc.format.extent217 p.en_US
dc.language.isoenen_US
dc.subjectDRNTU::Science::Biological sciences::Microbiology::Virologyen_US
dc.titleFunctional characterization of coronavirus non-structural proteinsen_US
dc.typeThesis
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.supervisorJames P. Tam
dc.contributor.supervisorLiu Ding Xiangen_US
dc.description.degreeDOCTOR OF PHILOSOPHY (SBS)en_US
dc.identifier.doihttps://doi.org/10.32657/10356/39373


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