Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/39373
Title: Functional characterization of coronavirus non-structural proteins
Authors: Chen, Bo
Keywords: DRNTU::Science::Biological sciences::Microbiology::Virology
Issue Date: 2008
Source: Chen, B. (2008). Functional characterization of coronavirus non-structural proteins. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: Genetic manipulation of the RNA genomes by reverse genetics is a powerful tool to study the molecular biology and pathogenesis of RNA viruses. During construction of an infectious clone from a Vero cell-adapted coronavirus infectious bronchitis virus (IBV), we found that a G - C point mutation at nucleotide position 15526, causing Arg-to-Pro mutation at amino acid position 132 of the helicase protein, is lethal to the infectivity of IBV on Vero cells. When the in vitro-synthesized full-length transcripts containing this mutation were introduced into Vero cells, no infectious virus was rescued. Upon correction of the mutation, infectious virus was recovered. Further characterization of the in vitro-synthesized full-length transcripts containing the G15526C mutation demonstrated that this mutation may block the transcription of subgenomic RNAs.
URI: https://hdl.handle.net/10356/39373
DOI: 10.32657/10356/39373
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Theses

Files in This Item:
File Description SizeFormat 
ChenBo08.pdf68.05 MBAdobe PDFThumbnail
View/Open

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.