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|Title:||Functional characterization of coronavirus non-structural proteins||Authors:||Chen, Bo||Keywords:||DRNTU::Science::Biological sciences::Microbiology::Virology||Issue Date:||2008||Source:||Chen, B. (2008). Functional characterization of coronavirus non-structural proteins. Doctoral thesis, Nanyang Technological University, Singapore.||Abstract:||Genetic manipulation of the RNA genomes by reverse genetics is a powerful tool to study the molecular biology and pathogenesis of RNA viruses. During construction of an infectious clone from a Vero cell-adapted coronavirus infectious bronchitis virus (IBV), we found that a G - C point mutation at nucleotide position 15526, causing Arg-to-Pro mutation at amino acid position 132 of the helicase protein, is lethal to the infectivity of IBV on Vero cells. When the in vitro-synthesized full-length transcripts containing this mutation were introduced into Vero cells, no infectious virus was rescued. Upon correction of the mutation, infectious virus was recovered. Further characterization of the in vitro-synthesized full-length transcripts containing the G15526C mutation demonstrated that this mutation may block the transcription of subgenomic RNAs.||URI:||https://hdl.handle.net/10356/39373||DOI:||10.32657/10356/39373||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Theses|
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