Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/39404
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dc.contributor.authorLim, Therese Shi Min.-
dc.date.accessioned2010-05-24T01:05:42Z-
dc.date.available2010-05-24T01:05:42Z-
dc.date.copyright2010en_US
dc.date.issued2010-
dc.identifier.urihttp://hdl.handle.net/10356/39404-
dc.description.abstractDepletion of natural energy resources and global climate change has renewed much interest in biofuels as an alternative form of energy. Isopropanol is both a desired fuel and essential chemical feedstock in the petrochemical industry. It is produced from fermentation using biocatalysts. Here in this work, we used directed evolution method to mutate the global transcription factor, cAMP Receptor Protein (CRP), to engineer isopropanol tolerance in Escherichia Coli (E. Coli). This has significant importance in increasing cell growth in high isopropanol concentrations and hence improving industrial production of isopropanol. In this study, a total of 3 different mutants were obtained. Each of the mutants has at least one mutation site adjacent to or inside the cAMP/ DNA binding pocket of the CRP protein. Also, all mutants were able to achieve about twice or more cell number concentrations than the wild-type cells. However, it was noted that the mutants have got shorter life spans due to shorter lag and exponential growth phases.en_US
dc.format.extent54 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University-
dc.subjectDRNTU::Engineering::Chemical engineering::Biotechnological productionen_US
dc.titleInceasing isopropanol tolerance in escherichia coli using randomized libraries of camp receptor proteinen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorJiang Rongrongen_US
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.description.degreeBachelor of Engineering (Chemical and Biomolecular Engineering)en_US
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Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)
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