Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/39638
Title: Bio-sorption of proteins
Authors: Chua, John Tze Yong.
Keywords: DRNTU::Engineering
Issue Date: 2010
Abstract: The aim of this project is to investigate the feasibility of adsorption of lysozyme from aqueous solution onto chitosan hydrogel beads and its derivatives. In this project, native chitosan hydrogel beads, GLU-chitosan beads and ECH-chitosan beads and are tested for adsorption of lysozyme. Crosslinking ratios of 0.2, 0.5, 0.7 and 1.0 are used for each chitosan hydrogel derivative across pH range of 4, 5, 5.5, 6, 9 and 10.5. All three types of chitosan beads are able to adsorb lysozyme. The adsorption isotherm data for adsorption of lysozyme at various pH were fitted to non-linear Langmuir isotherm model. The study showed low conformity to non linear Langmuir isotherm model of lysozyme onto all three types of chitosan beads at acidic pH range and high conformity to non linear Langmuir isotherm model of lysozyme onto all three types of chitosan beads near the isoelectric point of lysozyme(pH 10.9). SEM images shows high adsorption for all three types of beads at high alkaline region. The maximum adsorption capacity was the highest for all three type of beads at pH 10.5 with 306.25mg/g for native chitosan hydrogel beads, 515mg/g for ECH-Chitosan beads and 249mg/g for GLU-Chitosan beads
URI: http://hdl.handle.net/10356/39638
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)

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