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Title: Molecular cloning, expression, purification and characterization of a recombinant FKBP family member from Pseudomonas aeruginosa.
Authors: Tay, Shawn Wei Liang.
Keywords: DRNTU::Science::Biological sciences::Biochemistry
Issue Date: 2010
Abstract: The FK-506 binding proteins (FKBPs) are a family of proteins with characterised molecular functions such as peptidylprolyl cis-trans isomerase activity and chaperone activity. However, the FKBP family member from Pseudomonas aeruginosa is currently uncharacterised. This study has successfully developed a recombinant method of overexpressing and purifying the FK-506 binding domain of P. aeruginosa (PaFKBD) in a SUMO-PaFKBD fusion protein state. The characterisation of SUMO-PaFKBD revealed peptidylprolyl cis-trans isomerase activity with catalytic efficiency of 1.01 x 105 s-1 M-1 and this activity is inhibited by FK-506 with an IC50 of 92.6nM. More studies need to be done to further reaffirm these results and to further characterise the protein.
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)

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