Study of decellularization methods and characterization of porcine esophagus.
Date of Issue2008
School of Mechanical and Aerospace Engineering
BioMedical Engineering Research Centre
In order to reconstruct esophagus due to congenital or acquired esophageal diseases, a number of materials and techniques in tissue engineering have been developed to produce biocompatible and biodegradable scaffolds to support esophageal regeneration. Recently, acellular extracellular matrix (ECM) prepared by cell removal from native tissues (a novel technique called "decellularization") has shown promising results in regeneration of tissues such as skin, blood vessels and gastrointestinal tracts. Many decellularization methods have been developed but the effects of the methods on the resulting decellularized ECMs have not been fully elucidated. The aim of this project is to study two different decellularization protocols for producing decellularized porcine esophageal ECMs.