Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/41761
Title: Understanding the response heterogeneity of different cancer cell types toward anti-mitotic drugs I-Taxol
Authors: Tan, Adeline Hui Chen
Keywords: DRNTU::Science::Biological sciences::Microbiology::Drug Resistance
Issue Date: 2010
Abstract: Different types of cancer cells respond differently to anti-mitotic drug Taxol, resulting in its success and failure in its combat against cancer. Failure in the form of resistance of cancer cells to Taxol remains a huge obstacle for effective cancer treatment and for patients’ survival. In this study, we proposed that cellular proteins are the cause of the response heterogeneity. To identify the proteins of interest, cell lines HeLa cells and Taxol-resistant A549 cells were used for drug treatment, Bradford protein assay, SDS-PAGE and mass spectrometry. Western blotting was performed to ensure accuracy of the SDS-PAGE results. From the extensive MS analysis results, three proteins, Plectin, 78 kDa glucose-regulated protein precursor and Cullin-4B, were successfully shortlisted and further research studies are necessary to confirm that they are the proteins of interest.
URI: http://hdl.handle.net/10356/41761
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
Copy_of_Thesis_COMPLETE.pdf
  Restricted Access
474.58 kBAdobe PDFView/Open

Page view(s) 50

276
checked on Oct 20, 2020

Download(s) 50

12
checked on Oct 20, 2020

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.