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dc.contributor.authorLee, Sean Mun Kit.-
dc.description.abstractMyxobacteria are prokaryotes found commonly in soil that produces fruiting bodies and a diverse array of secondary metabolites. Tubulysins are highly potent anticancer compounds that are produced by most myxobacteria. Tubulysins disrupt microtubule networks of cancer cells leading to their inability to replicate and eventually cell death. A variety of myxobacteria strains were isolated from all three suborders of myxobacteria in the soils in Singapore. Metabolites from four isolated strains had cytotoxic effect on HeLa cells after incubation for 72 hours. Their crude extracts caused the diminishing of fluorescence of tubulins after 5 hours incubation and had greater effect after 24 hours incubation. HPLC showed high intensity peaks from 20 minutes to around 80 minutes. Mass spectrometry results revealed the presence of peaks which could be derivatives of pre-tubulysin A (pre-tub A) and tubulysin D (tub D). All four strains happened to belong to the suborder of Cystobacterineae, which is the reported tubulysin-producing suborder. This provided a basis for future research to be done with these four strains for the isolation of potential tubulysins to be used in cancer therapy.en_US
dc.format.extent47 p.en_US
dc.rightsNanyang Technological University-
dc.subjectDRNTU::Science::Biological sciences::Microbiology::Bacteriaen_US
dc.titleIsolation of tubulysins from myxobacteria.en_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorLiu Chuan Faen_US
dc.contributor.supervisorZhang Xiaohongen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeBachelor of Science in Biomedical Sciencesen_US
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Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)
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