Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/44620
Title: Construction and application of adenoviral and lentiviral vectors to deliver transforming growth factor β3 and type I collagen-targeting SHRNA for engineered articular chondrogenesis
Authors: Zhang, Feng
Keywords: DRNTU::Science::Medicine::Tissue engineering
DRNTU::Science::Biological sciences::Microbiology::Virology
Issue Date: 2011
Source: Zhang, F. (2011). Construction and application of adenoviral and lentiviral vectors to deliver transforming growth factor β3 and type I collagen-targeting SHRNA for engineered articular chondrogenesis. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: In this dissertation, we aim to induce type I collagen (Col I)-suppressed chondrogenesis in synovium-derived mesenchymal stem cells (SMSCs) or chondrocytes within 3-dimensional (3D) alginate hydrogel system with the use of adenoviral and/or lentiviral vectors to deliver both transforming growth factor β3 (TGF-β3) and Col I-targeting short hairpin RNA (shRNA). A collection of single-functioning and dual-functioning adenoviral/lentiviral vectors that express TGF-β3 and/or Col I-targeting shRNA were constructed and tested in SMSCs and chondrocytes encapsulated in 3D alginate hydrogel for Col I-suppressed chondrogenesis. One dual-functioning lentiviral vector with particular transgene arrangement (LV-1 in Chapter 4), and the combination of TGF-β3-expressing lentiviral vector and shRNA-encoding adenoviral vector (LV-T+Ad-sh in Chapter 5) were found to be relatively more effective than others in inducing chondrogenesis in SMSCs. Besides, the above dual-functioning lentiviral vector was also effective in inducing chondrocyte redifferentiation in 3D alginate hydrogel. These results suggest the promising potential of these viral vectors for the engineering of articular cartilage.
URI: http://hdl.handle.net/10356/44620
metadata.item.grantfulltext: restricted
metadata.item.fulltext: With Fulltext
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