Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/45128
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dc.contributor.authorPham Song Khanh Hang.
dc.date.accessioned2011-06-09T04:28:44Z
dc.date.available2011-06-09T04:28:44Z
dc.date.copyright2011en_US
dc.date.issued2011
dc.identifier.urihttp://hdl.handle.net/10356/45128
dc.description.abstractBeing the best understood DNA binding proteins, Zinc finger proteins can recognize and discriminate closely related sequences in both vitro and vivo (Klug, 2010). This ability has made them to be a promising candidate in creating a system that can detect DNAs in their native forms. In more than two years, the group of Professor Johnsson, EPFL, Switzerland, has been trying to develop a fusion protein between Zif 268, a Zinc finger protein, and two Snap-tags, which are useful self-labeling tool. Such protein, when catenating with a Zif-target containing plasmid, is believed to have many good biological applications. Unfortunately, the successful catenation reaction was observed to have low efficiency and selectivity due to dimensional problems. In this project, we aimed to introduce two elastic unstructured linkers to two locations between Zif268 and each Snap-tag to overcome such hindrance matter. A new protein with linker was highly expressed; however, its catenation efficiency seemed to be unchanged.en_US
dc.format.extent32 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Science::Biological sciences::Geneticsen_US
dc.titleDecorating dsDNA with a functional belt : bisAGT zif-finger protein for site-specific tagging of DNA.en_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeBachelor of Science in Biological Sciencesen_US
dc.contributor.organizationLab of Protein Engineering, EPFL, Switzerlanden_US
dc.contributor.supervisor2Kai Johnssonen_US
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Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)
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