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Title: Synaptotagmin genes expression profiling in single pancreatic α and β-cell.
Authors: Tan, Sijie.
Keywords: DRNTU::Science::Biological sciences::Genetics
Issue Date: 2011
Abstract: Gene expressions of synaptotagmins were characterized up to the resolution of pancreatic islets till date. However, it is unknown whether the synaptotagmin expressions identified were the results of α and/or β-cells transcription activities. Hence, we used a two-cycle multiplex and semi-nested PCR amplification procedure to first identify gene expressions of synaptotagmin isoforms I-X in mouse single pancreatic pseudo-islet, then increasing the resolution to single pancreatic α and β-cell. Our study detected the gene expressions of synaptotagmins V, VII and IX at the pseudo-islet level, and demonstrated that α-cells express synaptotagmins VII and IX, while β-cells express synaptotagmin VII. Based on the transcript abundance of synaptotagmin isoforms, we suggest that synaptotagmin VII is the principal Ca2+ sensor in Ca2+-dependent glucagon and insulin exocytosis in α and β-cells. We also characterized the gene expressions of synaptotagmin isoforms I-X in αTC-1 cells subjected to prolonged hyperglycemia, as a model to study synaptotagmin genes expression in pancreatic α-cells in Type II Diabetes Mellitus. We demonstrated that synaptotagmins III and VII genes expression were significantly up-regulated during hyperglycemia. Hence, we propose a hyperglycemia-induced stimulus-secretion coupling model in αTC-1 cells to account for this behaviour.
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)

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