Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/45387
Full metadata record
DC FieldValueLanguage
dc.contributor.authorLow, Darren Yong Yew.
dc.date.accessioned2011-06-13T04:20:57Z
dc.date.available2011-06-13T04:20:57Z
dc.date.copyright2011en_US
dc.date.issued2011
dc.identifier.urihttp://hdl.handle.net/10356/45387
dc.description.abstractRoots respond to various environmental stimuli and water in one of them. Under water stress, roots re-orientate and grow towards the side with higher water potential and this is known as hydrotropism. Regulatory molecules such as abscisic acid (ABA) and auxin play a important role in hydrotropism and expression of 9-cis-epoxycarotenoid dioxygenase (NCED) is needed for ABA biosynthesis. Presently, the molecular mechanisms of hydrotropism remain elusive. In this study, we wanted to analyze hydrotropism-deficient Arabidopsis mutants and discover more hydrotropism genes using our present understanding of hydrotropism. Moisture affinity assay and PG1 screening were conducted and many possible mutants of hydrotropism were found. We tried generating 16 more reporter lines for the 16 possible hydrotropism genes found in a prior study but did not manage to generate a single one due to some hiccups in the vector cloning. NCED3p-GFP screening was also done to prepare a NCED3 reporter line for hydrotropism studies. The complementation of the generation of the new reporter lines and the analysis of hydrotropic mutants will definitely help in identifying regulatory molecules and finding links between them. This study has given an taste of what greater discoveries are to come in the scientific research of plant hydrotropism.en_US
dc.format.extent37 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Science::Biological sciences::Botany::Plant ecologyen_US
dc.titleStudying the regulatory mechanisms controlling hydrotropism and moisture regulated gene expression in roots.en_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeBachelor of Science in Biological Sciencesen_US
dc.contributor.supervisor2José R. Dinnenyen_US
item.fulltextWith Fulltext-
item.grantfulltextrestricted-
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)
Files in This Item:
File Description SizeFormat 
BS119.pdf
  Restricted Access
878.06 kBAdobe PDFView/Open

Page view(s) 50

251
Updated on Jan 26, 2021

Download(s)

4
Updated on Jan 26, 2021

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.