Please use this identifier to cite or link to this item:
Title: Fiber-optic sensors for cell culture monitoring.
Authors: Loo, Quan Chun.
Keywords: DRNTU::Engineering::Bioengineering
Issue Date: 2011
Abstract: An optical fibre-based surface plasmon resonance glucose sensor design is proposed based on the optical properties of colloidal gold nanoparticles. In the proposed design, the colloidal surface is functionalised with glucose oxidase to provide selectivity for the sensor. A flow perfusion cell culture system was also developed, consisting of a customised bioreactor, peristaltic pump, and media reservoir. Cell culture experiments were done to compare cell viability in static and perfusion cultures as well as cell behaviour in 2D cultures compared to that of 3D cultures. Cell viability was shown to be higher in perfusion cultures and 3D cultures exhibited cell colonies. Integration of the SPR sensor and the flow perfusion system could potentially create a simple and low cost real-time optical fibre-based bio-sensing system.
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
  Restricted Access
1.42 MBAdobe PDFView/Open

Google ScholarTM


Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.