Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/45648
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dc.contributor.authorCheah, Petrie Jiing Ei.
dc.date.accessioned2011-06-15T09:25:08Z
dc.date.available2011-06-15T09:25:08Z
dc.date.copyright2011en_US
dc.date.issued2011
dc.identifier.urihttp://hdl.handle.net/10356/45648
dc.description.abstractThe main aim of drug delivery in cancer cell is to deliver anti-cancer drug agent that specifically and efficiently targeted to cancer cell. In this study, a biodegradable microcarrier system that utilized the Layer-by-Layer self assembled polyelectrolyte technique as drug delivery system and targeted to human breast cancer cell (MCF-7 & MCF-10a) will be investigated. This PEM systems include the CaCO3 microparticles as template core because of its advantage of biodegradability, biocompatibility and high adsorption ability. It is prepared by alternate coating with positively and negatively charged biodegradable polyelectrolyte (PRM/DSX) and fluorescence labeled with PAH-FITC and PRM-RITC. The result in terms of qualitative and quantitative measurement was promising. The observations and investigations were carried by means of Zeta Potential measurement, Confocal Laser Scanning Microscopy (CLSM), and Flow Cytometry (FCM). The cellular uptake was visualized using membrane stain and lysosomal activity was examined using lysotracker staining. This system was successfully internalized in cells and observed to increase in lysosomal activity. The saturated particle feed ratio was observed in between 1:5 and 1:10. Other than that, the activity of intracellular cysteine protease capthesins B in cell was observed via Immunocytochemistry assay and visualized under CLSM which will extend the project into the incorporation of cystatin, the cysteine protease inhibitors in CaCO3 PEMP in future.en_US
dc.format.extent64 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Engineering::Chemical engineering::Biotechnologyen_US
dc.titleFabrication of a biodegradable microcarrier system for drug delivery to cancer cells.en_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorBjoern Holger Neuen_US
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.description.degreeBachelor of Engineering (Chemical and Biomolecular Engineering)en_US
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Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)
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