Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/45736
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dc.contributor.authorLim, Carine Zhi Jun.-
dc.date.accessioned2011-06-16T08:28:01Z-
dc.date.available2011-06-16T08:28:01Z-
dc.date.copyright2011en_US
dc.date.issued2011-
dc.identifier.urihttp://hdl.handle.net/10356/45736-
dc.description.abstractThis study explores the utility of calcium-alginate hydrogels as a three-dimensional culture platform for hematopoietic cell culture. We investigate the effects of calcium concentration and alginate viscosity on the mechanical and physical properties of the gel, and their subsequent effects on cellular growth. Further, we have also explored the establishment of a co-culture system in the presence of stromal cells (HS-5) and using K562 cells as our hematopoietic model. Physical characterization of calcium alginate is conducted with mechanical testing and Scanning Electron Microscope (SEM) scans to determine the relationship between the mechanical strength and the structure of calcium alginate with the variation of calcium concentration and alginate viscosity. The study on mechanical properties via compressive stress test reveals that increasing calcium concentration and alginate viscosity resulted in the formation of stiffer gels as showed by the higher elastic moduli. In addition, SEM scans reveal increased density of surface folds with increasing calcium concentration.en_US
dc.format.extent92 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University-
dc.subjectDRNTU::Engineering::Bioengineeringen_US
dc.titleDesigning calcium alginate for ex vivo expansion of hematopoietic stem cells.en_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.description.degreeBachelor of Engineering (Chemical and Biomolecular Engineering)en_US
dc.contributor.supervisor2Mayasari Limen_US
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Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)
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