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Title: Designing calcium alginate for ex vivo expansion of hematopoietic stem cells.
Authors: Lim, Carine Zhi Jun.
Keywords: DRNTU::Engineering::Bioengineering
Issue Date: 2011
Abstract: This study explores the utility of calcium-alginate hydrogels as a three-dimensional culture platform for hematopoietic cell culture. We investigate the effects of calcium concentration and alginate viscosity on the mechanical and physical properties of the gel, and their subsequent effects on cellular growth. Further, we have also explored the establishment of a co-culture system in the presence of stromal cells (HS-5) and using K562 cells as our hematopoietic model. Physical characterization of calcium alginate is conducted with mechanical testing and Scanning Electron Microscope (SEM) scans to determine the relationship between the mechanical strength and the structure of calcium alginate with the variation of calcium concentration and alginate viscosity. The study on mechanical properties via compressive stress test reveals that increasing calcium concentration and alginate viscosity resulted in the formation of stiffer gels as showed by the higher elastic moduli. In addition, SEM scans reveal increased density of surface folds with increasing calcium concentration.
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)

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