dc.contributor.authorPasunooti, Kalyan Kumar
dc.date.accessioned2011-12-06T04:36:43Z
dc.date.accessioned2017-07-23T08:43:26Z
dc.date.available2011-12-06T04:36:43Z
dc.date.available2017-07-23T08:43:26Z
dc.date.copyright2011en_US
dc.date.issued2011
dc.identifier.citationPasunooti, K. K. (2011). Synthesis of bioactive amino acid building blocks and their applications towards the peptides and glycopeptides. Doctoral thesis, Nanyang Technological University, Singapore.
dc.identifier.urihttp://hdl.handle.net/10356/46460
dc.description.abstractSince the discovery of native chemical ligation (NCL) by Kent and coworkers in 1994, the field of peptide synthesis has been achieved significant advancement over the last two decades. This study demonstrated that the reaction undergoes a chemoselective ligation with unprotected peptides and proteins. Inspired by Dawson’s NCL followed by desulfurization strategy, several other research groups explored the native chemical ligation concept. In 2007, Crich and co-workers demonstrated “native chemical ligation at Phenylalanine”. Considerable efforts have been made on extending the applicability of NCL on various amino acid residues. Another successful native chemical ligation/desulfurization strategy was demonstrated on Valine by Seitz and Danishefsky groups in 2008. These two groups extended the scope of the native chemical ligation strategy using thiol containing Valine as a sulfur source. Based on previous investigations, we successfully synthesized lysine building blocks and expanded NCL concept to lysine and expanded application to synthesize ubiqutin and diubiquitin proteins. Further, we are extending the NCL strategy to aspartic acid (Asp) residue which can be utilized in N-linked glycopeptide synthesis. These N-linked glycopeptides are useful building blocks for glycopeptide-based vaccines (HIV-1 entry inhibitor). We have chosen a new synthetic strategy which combines the benefits of SPPS and convergent on-resin peptide synthesis to N-glycopeptides. Conventional synthetic approaches to N-glycopeptides lengthy and inefficient.en_US
dc.format.extent277 p.en_US
dc.language.isoenen_US
dc.subjectDRNTU::Science::Chemistry::Biochemistryen_US
dc.titleSynthesis of bioactive amino acid building blocks and their applications towards the peptides and glycopeptidesen_US
dc.typeThesis
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen_US
dc.contributor.supervisorLiu Xueweien_US
dc.description.degreeDOCTOR OF PHILOSOPHY (SPMS)en_US


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